Roberts R, Sobel B E, Parker C W
Science. 1976 Nov 19;194(4267):855-7. doi: 10.1126/science.982049.
Creatine kinase has three isoenzymes designated MM, MB, and BB, with BB being the grain form and MM the muscle form. Antibodies to BB creatine kinase were obtained by immunization of rabbits with human BB creatine kinase. The antibodies demonstrated specificity for BB and MB creatine kinase (myocardial isoenzyme) but no cross-reactivity with MM creatine kinase. With the use of this antibody, a highly sensitive radioimmunoassay capable of measuring picomolar amounts of MB creatine kinase has been developed. Clinical application of this method should provide a sensitive and specific test for the diagnosis of myocardial infarction.
肌酸激酶有三种同工酶，分别命名为MM、MB和BB，其中BB为脑型，MM为肌型。通过用人BB肌酸激酶免疫兔子获得了抗BB肌酸激酶抗体。这些抗体对BB和MB肌酸激酶（心肌同工酶）具有特异性，但与MM肌酸激酶无交叉反应。利用这种抗体，已开发出一种能够测量皮摩尔量MB肌酸激酶的高度灵敏的放射免疫测定法。该方法的临床应用应为心肌梗死的诊断提供一种灵敏且特异的检测方法。
