Vachhrajani K D, Dutta K K
Industrial Toxicology Research Centre, Lucknow, India.
Indian J Exp Biol. 1992 Oct;30(10):892-6.
Stage specific effect of single oral dose (500 mg/kg body wt) of ethylene glycol monomethyl ether (EGME) was characterised during one cycle of seminiferous epithelium in rats. Maximum peritubular membrane damage and germinal epithelial distortion were observed at stages IX-XII. Cell death occurred during conversion of zygotene to pachytene spermatocytes (stage XIII) and between dividing spermatocytes and step I spermatids (stage late XIII-XIV). Profound effect was noted during first meiotic division than during second meiotic division. Presence of multinucleated secondary spermatocytes indicated cytokinesis arrest. The spermatogenesis was delayed and consequently frequency of tubules at stages I-VIII was reduced by day 10. Many of the tubules were devoid of round spermatids on day 12. Possibly, EGME (or it's metabolite) distorted the barrier system at stages IX-XIV and damaged the cells mostly at stages XII-early XIV.
在大鼠生精上皮的一个周期中，对单剂量口服（500毫克/千克体重）乙二醇单甲醚（EGME）的阶段特异性效应进行了表征。在IX - XII期观察到最大的睾丸周膜损伤和生精上皮畸变。细胞死亡发生在偶线期向粗线期精母细胞转化期间（XIII期）以及分裂期精母细胞和I期精子细胞之间（XIII期末 - XIV期）。第一次减数分裂期间的影响比第二次减数分裂期间更为显著。多核次级精母细胞的存在表明胞质分裂停滞。精子发生延迟，因此到第10天时，I - VIII期的生精小管频率降低。在第12天时，许多生精小管中没有圆形精子细胞。可能是EGME（或其代谢产物）在IX - XIV期破坏了屏障系统，并主要在XII - XIV早期损伤了细胞。
