Sillman Amy L, Quang Dung My, Farboud Benhom, Fang Kathy S, Nuccitelli Richard, Isseroff R Rivkah
Department of Dermatology, University of California, Davis, CA, USA.
Exp Dermatol. 2003 Aug;12(4):396-402. doi: 10.1034/j.1600-0625.2002.120406.x.
Endogenous electric fields are generated lateral to skin wounds, with the cathodal pole of the field residing in the center of the wound. These fields are thought to be an important mechanism in guiding the migration of keratinocytes and other cells into wounds to effect healing. In this work, human dermal fibroblasts were exposed to direct current electric fields of physiological strength, and their migrational behavior was quantitated. Only random migration of human dermal fibroblasts was observed in direct-current electric fields under conditions that support the directional migration of human epidermal keratinocytes. Additionally, neither the presence of serum nor serum plus additional Mg++ in the experimental medium supported directional migration. Migratory rates of fibroblasts varied depending on the experimental medium used: in serum-containing medium the average velocity was as low as 0.23 micro m/min, while in serum-free keratinocyte medium the average velocity was as high as 0.36 micro m/min. These studies suggest that dermal fibroblasts do not respond to the endogenous electric field of a wound, and use other migratory cues to direct their movement into the wound bed.
内源性电场在皮肤伤口外侧产生，电场的阴极位于伤口中心。这些电场被认为是引导角质形成细胞和其他细胞迁移到伤口以实现愈合的重要机制。在这项研究中，将人类真皮成纤维细胞暴露于生理强度的直流电场中，并对其迁移行为进行定量分析。在支持人类表皮角质形成细胞定向迁移的条件下，在直流电场中仅观察到人类真皮成纤维细胞的随机迁移。此外，实验培养基中血清的存在或血清加额外的Mg++均不支持定向迁移。成纤维细胞的迁移速率因所用实验培养基而异：在含血清的培养基中，平均速度低至0.23μm/分钟，而在无血清角质形成细胞培养基中，平均速度高达0.36μm/分钟。这些研究表明，真皮成纤维细胞对伤口的内源性电场无反应，并利用其他迁移线索引导其移动到伤口床。
