Cong Yu-long, Jin Da-ming, Wang Hong-li, Okada Tokuhiro, Peng Zuo-hui
Department of Clinical Laboratory, Chinese PLA General Hospital, Beijing 100853, China.
Zhonghua Yi Xue Za Zhi. 2003 Jul 25;83(14):1201-5.
To establish the reference range of venous blood measured by automated hematology analyzer in China.
Different hematoanalyzers were used to examine the white blood cell (WBC), red blood cell (RBC), hemoglobin (Hb), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular volume (MCV), and hemotocrit (HCT) of venous blood from 1,749 healthy people, 927 males and 822 females, aged 18 - 60, in 14 cities (Harbin, Changchun, Beijing, Tianjin, Lanzhou, Xi'an, Nanjing, Suzhou, Chengdu, Wuhan, Chongqing, Fuzhou, Kunming, and Guangzhou) of China. The examination was completed within the period of one month under strict in-laboratory quality control.
The range of WBC was (3.97 - 9.15) x 10(9)/L in males and (3.69 - 9.16) x 10(9)/L in females. The range of RBC was (4.09 - 5.74) x 10(12)/L in males and (3.68 - 5.13) x 10(12)/L in females. The range Hb was 131 - 172 (151) g/L in males and 113 - 151 (129) g/L in females. The range of MCH was 27.8 - 33.8 (30.8) pg in males and 26.9 - 33.3 (30.2) pg in females. The range of MCHC was 263 - 375 (335) g/L in males and 278 - 372 (325) g/L in females. The range of MCV was 83.9 - 99.1 (91.2) fl in males and 82.6 - 99.1 (91.3) fl in females. The range of HCT was 38.0% - 50.8% (44.8%) in males and 33.5% - 45.0% (38.9%) in females. The lower limits of WBC in Lanzhou and Chengdu were lower than those in other cities (P < 0.01). The concentrations of RBC and Hb in Kunming were significantly higher than those in other cities (P < 0.01). There was no significant difference in other parameters among different cities. The WBC, RBC, and Hb in the females were lower than those in the males (P < 0.01).
The reference ranges of different parameters of venous blood of Chinese have been primarily established. There is little discrepancy in those reference range in different district (except for highland). Some of the indices such as WBC, MCH and MCV measured by analyzer are quite different from those measured manually.
建立我国自动血液分析仪测定静脉血的参考范围。
采用不同血液分析仪检测来自中国14个城市（哈尔滨、长春、北京、天津、兰州、西安、南京、苏州、成都、武汉、重庆、福州、昆明和广州）18至60岁的1749名健康人（927名男性和822名女性）静脉血中的白细胞（WBC）、红细胞（RBC）、血红蛋白（Hb）、平均红细胞血红蛋白含量（MCH）、平均红细胞血红蛋白浓度（MCHC）、平均红细胞体积（MCV）和血细胞比容（HCT）。在严格的实验室质量控制下，一个月内完成检测。
男性WBC范围为（3.97 - 9.15）×10⁹/L，女性为（3.69 - 9.16）×10⁹/L。男性RBC范围为（4.09 - 5.74）×10¹²/L，女性为（3.68 - 5.13）×10¹²/L。男性Hb范围为131 - 172（151）g/L，女性为113 - 151（129）g/L。男性MCH范围为27.8 - 33.8（30.8）pg，女性为26.9 - 33.3（30.2）pg。男性MCHC范围为263 - 375（335）g/L，女性为278 - 372（325）g/L。男性MCV范围为83.9 - 99.1（91.2）fl，女性为82.6 - 99.1（91.3）fl。男性HCT范围为38.0% - 50.8%（44.8%），女性为33.5% - 45.0%（38.9%）。兰州和成都的WBC下限低于其他城市（P < 0.01）。昆明的RBC和Hb浓度显著高于其他城市（P < 0.01）。不同城市间其他参数无显著差异。女性的WBC、RBC和Hb低于男性（P < 0.01）。
初步建立了我国静脉血不同参数的参考范围。不同地区（除高原地区外）这些参考范围差异不大。分析仪检测的一些指标如WBC、MCH和MCV与手工检测的有较大差异。
