Dai Ke-rong, Xu Xiao-liang, Tang Ting-ting, Zhu Zhen-an, Yu Chao-feng, Xu Min, Zhu Liu-long, Hao Yong-qiang, Lou Jue-ren
Department of Orthopaedics, Ninth People's Hospital, Shanghai Second Medical University, Shanghai 200011, China.
Zhonghua Yi Xue Za Zhi. 2003 Aug 10;83(15):1345-9.
To evaluate the effectiveness of the tissue-engineered bone substitute loaded with adenovirus mediated human bone morphogenetic protein-2 gene (Adv-hBMP-2) transfected bone marrow derived mesenchymal stem cells (BMSC) in the repair of diaphyseal segmental bone defect of large animal.
The right tibial bone defects (2.6 cm) model of 26 goats were established and divided into 5 groups: I. Adv-hBMP-2 transfected BMSC/calcined bone (CB) group (n = 9); II. adenovirus-beta-galactosidase (Adv-betagal) gene transfected BMSC/CB group (n = 6); III. untransfected BMSC/CB group (n = 6); IV. single CB group (n = 3); VI. untreated group (n = 2). The above tissue-engineered bone substitutes were implanted in the bone defects respectively except group VI. Roentgenography, histomorphometrical analysis and biomechanical measurement were studied at various times.
X-ray: at 4 - 8th weeks after implantation, more bony callus was found in the bone defects of group I. The complete healing rates of group I, II, III, IV, and V were 5/8, 1/5, 0/5, 0/2, 0/1 respectively at 26th week after implantation. Histomorphometrical analysis showed much more new bony callus including cortical bone formed in group I than those of other groups. The compression strength of the implanted bone substitute of group I is significantly higher than those of group II and III.
The tissue-engineered bone substitute loaded with human BMP-2 gene transfected BMSC can repair diaphyseal segmental bone defect of large animal (goat).
评估负载腺病毒介导的人骨形态发生蛋白-2基因（Adv-hBMP-2）转染的骨髓间充质干细胞（BMSC）的组织工程骨替代物在修复大型动物骨干节段性骨缺损中的有效性。
建立26只山羊的右胫骨骨缺损（2.6厘米）模型，并分为5组：I. Adv-hBMP-2转染的BMSC/煅烧骨（CB）组（n = 9）；II. 腺病毒-β-半乳糖苷酶（Adv-betagal）基因转染的BMSC/CB组（n = 6）；III. 未转染的BMSC/CB组（n = 6）；IV. 单纯CB组（n = 3）；V. 未处理组（n = 2）。除V组外，将上述组织工程骨替代物分别植入骨缺损处。在不同时间进行X线摄影、组织形态计量学分析和生物力学测量。
X线：植入后4 - 8周，I组骨缺损处发现更多骨痂。植入后第26周，I、II、III、IV和V组的完全愈合率分别为5/8、1/5、0/5、0/2、0/1。组织形态计量学分析显示，I组形成的包括皮质骨在内的新骨痂比其他组多得多。I组植入骨替代物的抗压强度明显高于II组和III组。
负载人BMP-2基因转染的BMSC的组织工程骨替代物可修复大型动物（山羊）骨干节段性骨缺损。
