Chen Bin, Pei Guo-xian, Wang Ke, Jin Dan, Wei Kuan-hai, Ren Gao-hong
Department of Orthopaedics and Traumatology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 2003 Feb;25(1):26-31.
To study whether tissue engineered bone can repair the large segment bone defect of large animal or not. To observe what character the fascia flap played during the osteanagenesis and revascularization process of tissue engineered bone.
9 Chinese goats were made 2 cm left tibia diaphyseal defect. The repairing effect of the defects was evaluated by ECT, X-ray and histology. 27 goats were divided into three groups: group of CHAP, the defect was filled with coral hydroxyapatite (CHAP); group of tissue engineered bone, the defect was filled with CHAP + bone marrow stroma cells (BMSc); group of fascia flap, the defect was filled with CHAP + BMSc + fascia flap. After finished culturing and inducing the BMSc, CHAP of group of tissue engineered bone and of fascia flap was combined with it. Making fascia flap, different materials as described above were then implanted separately into the defects. Radionuclide bone imaging was used to monitor the revascularization of the implants at 2, 4, 8 weeks after operation. X-ray examination, optical density index of X-ray film, V-G staining of tissue slice of the implants were used at 4, 8, 12 weeks after operation, and the biomechanical character of the specimens were tested at 12 weeks post operation.
In the first study, the defect showed no bone regeneration phenomenon. 2 cm tibia defect was an ideal animal model. In the second study, group of CHAP manifested a little trace of bone regeneration, as to group of tissue engineered bone, the defect was almost repaired totally. In group of fascia flap, with the assistance of fascia flap which gave more chance to making implants to get more nutrient, the repair was quite complete.
The model of 2 cm caprine tibia diaphyseal defect cannot be repaired by goat itself and can satisfy the tissue engineering's demands. Tissue engineered bone had good ability to repair large segment tibia defect of goat. Fascia flap can accelerate the revascularization process of tissue engineered bone. And by this way, it augment the ability of tissue engineered bone to repair the large bone defect of goat.
研究组织工程骨能否修复大型动物的大段骨缺损，观察筋膜瓣在组织工程骨成骨及血管化过程中所起的作用。
对9只中国山羊造成左胫骨骨干2cm缺损，通过ECT、X线及组织学方法评估缺损的修复效果。将27只山羊分为三组：CHAP组，缺损处填充珊瑚羟基磷灰石（CHAP）；组织工程骨组，缺损处填充CHAP+骨髓基质细胞（BMSc）；筋膜瓣组，缺损处填充CHAP+BMSc+筋膜瓣。在对BMSc进行培养和诱导后，将组织工程骨组和筋膜瓣组的CHAP与之复合，制作筋膜瓣，然后将上述不同材料分别植入缺损处。术后2、4、8周采用放射性核素骨显像监测植入物的血管化情况。术后4、8、12周进行X线检查、X线片光密度指数测定、植入物组织切片的V-G染色，并在术后12周测试标本的生物力学特性。
在第一项研究中，缺损处未出现骨再生现象。2cm胫骨缺损是理想的动物模型。在第二项研究中，CHAP组显示有少量骨再生迹象，而组织工程骨组缺损几乎完全修复。在筋膜瓣组，在筋膜瓣的辅助下，植入物有更多机会获得营养，修复相当完整。
2cm山羊胫骨骨干缺损模型山羊自身无法修复，能满足组织工程要求。组织工程骨具有良好的修复山羊大段胫骨缺损的能力。筋膜瓣可加速组织工程骨的血管化进程，从而增强组织工程骨修复山羊大骨缺损的能力。
