Domar U, Nilsson B, Baranov V, Gerdes U, Stigbrand T
Department of Medical Biochemistry and Biophysics, University of Umeå, Sweden.
Histochemistry. 1992 Dec;98(6):359-64. doi: 10.1007/BF00271071.
Human intestinal alkaline phosphatase was immunohistochemically identified and localized in the pancreas, liver and kidney by use of a monoclonal antibody specific for intestinal alkaline phosphatase isozyme and by amplified biotin-streptavidin staining. In all the examined organs, the intestinal isozyme was found to be localized in the epithelial cells of ducts: bile ducts in the liver, distal convoluted tubules and collecting tubules in the kidney and ducts in the secretory epithelium in the pancreas. In the liver the antibody also stained some sinus-lining cells. In all the examined organs the endothelial cells of the capillaries and some vessels were stained. By use of immunoelectron microscopy, intestinal alkaline phosphatase was, as expected, found to be localized to the microvillar region of the small intestine. The isozyme was abundantly expressed in the apical area of the microvilli and in membrane remnants in the fuzzy coat. Capillaries and vessels in the submucosa were also stained, as well as small vesicles in the endothelial cells. The present investigation demonstrates the expression and localization of the intestinal alkaline phosphatase in several organs, though previously believed to be expressed only in the intestine.
通过使用针对肠碱性磷酸酶同工酶的单克隆抗体以及生物素-链霉亲和素放大染色法，对人肠碱性磷酸酶进行免疫组织化学鉴定，并将其定位在胰腺、肝脏和肾脏中。在所有检查的器官中，发现肠同工酶定位于导管的上皮细胞：肝脏中的胆管、肾脏中的远曲小管和集合小管以及胰腺分泌上皮中的导管。在肝脏中，抗体还对一些窦周细胞进行了染色。在所有检查的器官中，毛细血管和一些血管的内皮细胞都被染色。通过免疫电子显微镜观察，正如预期的那样，发现肠碱性磷酸酶定位于小肠的微绒毛区域。该同工酶在微绒毛的顶端区域和模糊层中的膜残余物中大量表达。黏膜下层的毛细血管和血管以及内皮细胞中的小泡也被染色。本研究证明了肠碱性磷酸酶在几个器官中的表达和定位，尽管以前认为它仅在肠道中表达。
