Ono K
Acta Histochem. 1976;57(2):312-9.
The localization of alkaline phosphatase activity in the large intestinal mucosa of postnatal developing rats was studied by means of light and electron microscopes. On the day after birth, alkaline phosphatase activity, as shown by the azo-coupling method was found on the microvilli and the tubulovacuolar system in the proximal part of the large intestine between the 1st and the 15th days, enzyme activity was localized on the microvilli, the tubulovacuolar system, and the supranuclear vacuole of the surface principal cells of the cecum and the ascending colon. After the 15th day, all the principal cells of the proximal large intestine were free of reaction products as were those in the full-grown rats. Before the 4th day of life, in the descending colon, the enzyme activity of the principal cells was observed, but only on the microvilli. After the 5th day, enzyme activity disappeared from the principal cells.
通过光学显微镜和电子显微镜研究了出生后发育中大鼠大肠黏膜碱性磷酸酶活性的定位。出生后第一天，用偶氮偶联法显示，在大肠近端的微绒毛和小管泡系统上发现了碱性磷酸酶活性。在第1天至第15天之间，盲肠和升结肠表面主细胞的微绒毛、小管泡系统和核上空泡中有酶活性。第15天后，近端大肠的所有主细胞均无反应产物，成年大鼠的主细胞也是如此。在出生后第4天之前，在降结肠中观察到主细胞的酶活性，但仅在微绒毛上。第5天后，主细胞的酶活性消失。
