相似文献
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Promoter choice affects the potency of HIV-1 specific RNA interference.启动子的选择会影响HIV-1特异性RNA干扰的效力。
Nucleic Acids Res. 2003 Sep 1;31(17):5033-8. doi: 10.1093/nar/gkg704.
2
Human immunodeficiency virus type 1 escape from RNA interference.1型人类免疫缺陷病毒对RNA干扰的逃逸
J Virol. 2003 Nov;77(21):11531-5. doi: 10.1128/jvi.77.21.11531-11535.2003.
3
Enhanced gene silencing of HIV-1 specific siRNA using microRNA designed hairpins.使用微小RNA设计的发夹增强HIV-1特异性小干扰RNA的基因沉默作用。
Nucleic Acids Res. 2004 Feb 13;32(3):1154-8. doi: 10.1093/nar/gkh278. Print 2004.
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Efficient gene transfer of HIV-1-specific short hairpin RNA into human lymphocytic cells using recombinant adeno-associated virus vectors.利用重组腺相关病毒载体将HIV-1特异性短发夹RNA高效导入人淋巴细胞。
Mol Ther. 2004 Mar;9(3):396-402. doi: 10.1016/j.ymthe.2003.11.025.
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Retroviral vectors designed for targeted expression of RNA polymerase III-driven transcripts: a comparative study.用于RNA聚合酶III驱动转录本靶向表达的逆转录病毒载体：一项比较研究。
Gene. 1996 Jun 1;171(2):203-8. doi: 10.1016/0378-1119(96)00075-3.
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Optimization and characterization of tRNA-shRNA expression constructs.tRNA-短发夹RNA表达构建体的优化与表征
Nucleic Acids Res. 2007;35(8):2620-8. doi: 10.1093/nar/gkm103. Epub 2007 Apr 10.
7
HIV-1-specific RNA interference.HIV-1特异性RNA干扰
Curr Opin Mol Ther. 2004 Aug;6(4):373-80.
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The inhibitory efficacy of RNA POL III-expressed long hairpin RNAs targeted to untranslated regions of the HIV-1 5' long terminal repeat.靶向HIV-1 5'长末端重复序列非翻译区的RNA聚合酶III表达的长链发夹RNA的抑制效力。
Oligonucleotides. 2007 Winter;17(4):419-31. doi: 10.1089/oli.2007.0095.
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Novel Pol II fusion promoter directs human immunodeficiency virus type 1-inducible coexpression of a short hairpin RNA and protein.新型RNA聚合酶II融合启动子指导人免疫缺陷病毒1型诱导的短发夹RNA和蛋白质共表达。
J Virol. 2006 Feb;80(4):1863-73. doi: 10.1128/JVI.80.4.1863-1873.2006.
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Characterisation and application of a bovine U6 promoter for expression of short hairpin RNAs.用于短发夹RNA表达的牛U6启动子的表征与应用
BMC Biotechnol. 2005 May 11;5:13. doi: 10.1186/1472-6750-5-13.
引用本文的文献
1
Stable expression of shRNA for the control of recombinant adenovirus replication.稳定表达短发夹 RNA 以控制重组腺病毒复制。
Braz J Med Biol Res. 2023 Jul 21;56:e12682. doi: 10.1590/1414-431X2023e12682. eCollection 2023.
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A T7 autogene-based hybrid mRNA/DNA system for long-term shRNA expression in cytoplasm without inefficient nuclear entry.一种基于 T7 自动基因的杂交 mRNA/DNA 系统，可在细胞质中实现长期 shRNA 表达，而无需低效的核进入。
Sci Rep. 2019 Feb 28;9(1):2993. doi: 10.1038/s41598-019-39407-8.
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Are microRNAs Important Players in HIV-1 Infection? An Update.微小 RNA 是 HIV-1 感染的重要参与者吗？最新研究进展。
Viruses. 2018 Mar 3;10(3):110. doi: 10.3390/v10030110.
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Delineation of the Exact Transcription Termination Signal for Type 3 Polymerase III.III型RNA聚合酶精确转录终止信号的描绘
Mol Ther Nucleic Acids. 2018 Mar 2;10:36-44. doi: 10.1016/j.omtn.2017.11.006. Epub 2017 Nov 21.
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Expansion of the CRISPR-Cas9 genome targeting space through the use of H1 promoter-expressed guide RNAs.通过使用H1启动子表达的引导RNA扩展CRISPR-Cas9基因组靶向范围。
Nat Commun. 2014 Aug 8;5:4516. doi: 10.1038/ncomms5516.
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Transfer and Expression of Small Interfering RNAs in Mammalian Cells Using Lentiviral Vectors.利用慢病毒载体在哺乳动物细胞中转录和表达小干扰 RNA。
Acta Naturae. 2013 Apr;5(2):7-18.
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Human U6 promoter drives stronger shRNA activity than its schistosome orthologue in Schistosoma mansoni and human fibrosarcoma cells.人类 U6 启动子在曼氏血吸虫和人成纤维肉瘤细胞中比其直向同源物驱动更强的 shRNA 活性。
Transgenic Res. 2012 Jun;21(3):511-21. doi: 10.1007/s11248-011-9548-0. Epub 2011 Sep 28.
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Illuminating the gateway of gene silencing: perspective of RNA interference technology in clinical therapeutics.基因沉默的光明之门：RNA 干扰技术在临床治疗中的展望。
Mol Biotechnol. 2012 Jul;51(3):289-302. doi: 10.1007/s12033-011-9456-9.
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The effects of stem length and core placement on shRNA activity.茎长和核定位对 shRNA 活性的影响。
BMC Mol Biol. 2011 Aug 8;12:34. doi: 10.1186/1471-2199-12-34.
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RNA interference for improving the outcome of islet transplantation.利用 RNA 干扰技术改善胰岛移植的效果。
Adv Drug Deliv Rev. 2011 Jan-Feb;63(1-2):47-68. doi: 10.1016/j.addr.2010.11.003. Epub 2010 Dec 13.
本文引用的文献
1
Short hairpin type of dsRNAs that are controlled by tRNA(Val) promoter significantly induce RNAi-mediated gene silencing in the cytoplasm of human cells.由tRNA(Val)启动子控制的短发夹型双链RNA能在人细胞质中显著诱导RNA干扰介导的基因沉默。
Nucleic Acids Res. 2003 Jan 15;31(2):700-7. doi: 10.1093/nar/gkg158.
2
siRNA-mediated gene silencing in vitro and in vivo.体外和体内的小干扰RNA介导的基因沉默
Nat Biotechnol. 2002 Oct;20(10):1006-10. doi: 10.1038/nbt739. Epub 2002 Sep 16.
3
Stable suppression of tumorigenicity by virus-mediated RNA interference.病毒介导的RNA干扰对肿瘤发生的稳定抑制作用。
Cancer Cell. 2002 Sep;2(3):243-7. doi: 10.1016/s1535-6108(02)00122-8.
4
Potent and specific inhibition of human immunodeficiency virus type 1 replication by RNA interference.RNA干扰对1型人类免疫缺陷病毒复制的强效特异性抑制作用。
J Virol. 2002 Sep;76(18):9225-31. doi: 10.1128/jvi.76.18.9225-9231.2002.
5
RNA interference in human cells is restricted to the cytoplasm.人类细胞中的RNA干扰仅限于细胞质。
RNA. 2002 Jul;8(7):855-60. doi: 10.1017/s1355838202020071.
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A microRNA in a multiple-turnover RNAi enzyme complex.多轮循环RNA干扰酶复合物中的一种微小RNA。
Science. 2002 Sep 20;297(5589):2056-60. doi: 10.1126/science.1073827. Epub 2002 Aug 1.
7
Effective expression of small interfering RNA in human cells.小干扰RNA在人细胞中的有效表达。
Nat Biotechnol. 2002 May;20(5):505-8. doi: 10.1038/nbt0502-505.
8
Expression of small interfering RNAs targeted against HIV-1 rev transcripts in human cells.针对HIV-1 rev转录本的小干扰RNA在人细胞中的表达。
Nat Biotechnol. 2002 May;20(5):500-5. doi: 10.1038/nbt0502-500.
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Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells.短发夹RNA（shRNA）可在哺乳动物细胞中诱导序列特异性沉默。
Genes Dev. 2002 Apr 15;16(8):948-58. doi: 10.1101/gad.981002.
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A system for stable expression of short interfering RNAs in mammalian cells.一种用于在哺乳动物细胞中稳定表达小干扰RNA的系统。
Science. 2002 Apr 19;296(5567):550-3. doi: 10.1126/science.1068999. Epub 2002 Mar 21.
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