[Hela cells secrete interleukin-8 and interleukin-10 response to Chlamydia trachomatis entry].

OBJECTIVE

To investigate the effect of Chlamydia trachomatis serotype K infection on productions of IL-8 and IL-10 in supernatant of cultured Hela cells.

METHODS

The levels of IL-8 and IL-10 productions were detected by cell culture method and ELISA assay.

RESULTS

The amount of IL-8 and IL-10 in the cell culture supernatant rose with the increasing doses of Chlamydia trachomatis. The concentrations of IL-8 and IL-10 began to increase obviously at 24 h, and reached their highest level at 72 h, and then decreased. Heat-inactivated Chlamydia trachomatis induced lower levels of IL-8 and IL-10 production than live bacteria in vitro.

CONCLUSION

The Chlamydia trachomatis can induce the secretion of IL-8 and IL-10 of host cells in a dose and time dependent manner and the intracellar replication of the bacteria is required for enhancing IL-8 and IL-10 productions by the infected cells.