Jiang Li-ping, Li Yun-yi, Tong Wen-ge, Yao Ping, Song Yu-guo, Sha Jan-hui
Department of Physiology, Jilin Military Medical College, The Fourth Military Medical University, Jilin, China.
Space Med Med Eng (Beijing). 2003 Jun;16(3):220-2.
To study the effect of push-pull maneuver on cerebral apoptosis and NO content of rats.
Ninety male Wistar rats were randomly divided into the control group, +Gz exposure group and the push-pull maneuver group. The rats were killed after 30 min exposure, 3 h, 12 h, 24 h or 48 h exposure, and the brain tissues were taken. The variation of NO content measured was using chemical colormetry. Then flow cytometric method was adopted to examine the distribution diagram of cortex and hippocampus-cell DNA for determination of percentage of A0 peak, which could express apoptosis degree.
NO content in +Gz exposure group and push-pull group are significantly different from that of control at 30 min, 3 h and 12 h. And the difference between +Gz group and push-pull group are also significant, but not at 24 h and 48 h. Comparing with the control group, the apoptosis amount of cortex and hippocampus-cell of +Gz exposure group and push-pull maneuver group increased obviously at 3 h, 12 h and 24 h, reached its peak at 24 h, and had no remarkable difference at 48 h. The comparison of the apoptosis amount of cortex cell and hippocampus cell between push-pull maneuver group and +Gz exposure group, difference at 3 h and 24 h and remarkably different at 12 h.
High +Gz and push-pull maneuver may increase the percentage of cerebral apoptosis amount and NO content. Comparing with the +Gz exposure group, the push-pull maneuver group will cause more increase in apoptosis amount and NO content, but this damage is reversible.
研究推拉动作对大鼠脑凋亡及一氧化氮（NO）含量的影响。
将90只雄性Wistar大鼠随机分为对照组、+Gz暴露组和推拉动作组。暴露30分钟、3小时、12小时、24小时或48小时后处死大鼠，取脑组织。采用化学比色法测定NO含量变化。然后采用流式细胞术检测皮质和海马细胞DNA分布图，测定可表达凋亡程度的A0峰百分比。
+Gz暴露组和推拉动作组在30分钟、3小时和12小时时的NO含量与对照组相比有显著差异。+Gz组和推拉动作组之间也有显著差异，但在24小时和48小时时无差异。与对照组相比，+Gz暴露组和推拉动作组皮质和海马细胞的凋亡量在3小时、12小时和24小时时明显增加，在24小时时达到峰值，在48小时时无显著差异。推拉动作组与+Gz暴露组皮质细胞和海马细胞凋亡量比较，在3小时和24小时时有差异，在12小时时有显著差异。
高+Gz和推拉动作可能会增加脑凋亡量百分比和NO含量。与+Gz暴露组相比，推拉动作组会导致凋亡量和NO含量增加更多，但这种损伤是可逆的。
