Sickmann Albert, Mreyen Marcus, Meyer Helmut E
Rudolf-Virchow-Zentrum, DFG Forschungszentrum für Experimentelle Biomedizin, Versbacher Strasse 9, Raum 411, 97078 Würzburg, Germany.
Adv Biochem Eng Biotechnol. 2003;83:141-76. doi: 10.1007/3-540-36459-5_6.
The rapid developments in the field of mass spectrometry have transformed it into a key technology in proteome research. Increased sensitivity in mass spectrometry, as a result of more efficient ionisation techniques and better detectors, has allowed the stepwise reduction of protein quantity for analysis. Protein spots of 2D-PAGE separated samples are now quantitatively sufficient for an unequivocal identification of a protein by mass spectrometry. In addition to protein identification a closer look at posttranslational modifications is now also possible. It is speculated that modifications like phosphorylation or glycosylation exist on every second protein and that they are important for the protein function. This review highlights the different mass spectrometric methods and gives a brief overview of strategies and methods used to identify modifications.
质谱领域的快速发展已将其转变为蛋白质组研究中的一项关键技术。由于采用了更高效的电离技术和性能更佳的探测器，质谱的灵敏度得以提高，这使得分析所需的蛋白量逐步减少。二维聚丙烯酰胺凝胶电泳（2D-PAGE）分离样品中的蛋白质斑点现在在数量上已足以通过质谱明确鉴定一种蛋白质。除了蛋白质鉴定之外，现在还能够更深入地研究翻译后修饰。据推测，诸如磷酸化或糖基化之类的修饰存在于每两个蛋白质中，并且它们对蛋白质功能很重要。本综述着重介绍了不同的质谱方法，并简要概述了用于鉴定修饰的策略和方法。
