Yu Guo-Jun, Yin Ya-Lin, Yu Wen-Hui, Liu Wei, Jin Yan-Xia, Shrestha Alok, Yang Qing, Ye Xiang-Dong, Sun Hui
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China.
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan, China; Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Ministry of Education), Wuhan University, Wuhan, China; Hubei Provincial Cooperative Innovation Center of Industrial Fermentation, Key Laboratory of Fermentation Engineering (Ministry of Education), Hubei University of Technology, Wuhan, China.
PLoS One. 2015 Mar 10;10(3):e0119439. doi: 10.1371/journal.pone.0119439. eCollection 2015.
Ganoderma lucidum is a basidiomycete white rot fungus that has been used for medicinal purposes worldwide. Although information concerning its genome and transcriptome has recently been reported, relatively little information is available for G. lucidum at the proteomic level. In this study, protein fractions from G. lucidum at three developmental stages (16-day mycelia, and fruiting bodies at 60 and 90 days) were prepared and subjected to LC-MS/MS analysis. A search against the G. lucidum genome database identified 803 proteins. Among these proteins, 61 lignocellulose degrading proteins were detected, most of which (49 proteins) were found in the 90-day fruiting bodies. Fourteen TCA-cycle related proteins, 17 peptidases, two argonaute-like proteins, and two immunomodulatory proteins were also detected. A majority (470) of the 803 proteins had GO annotations and were classified into 36 GO terms, with "binding", "catalytic activity", and "hydrolase activity" having high percentages. Additionally, 357 out of the 803 proteins were assigned to at least one COG functional category and grouped into 22 COG classifications. Based on the results from the proteomic and sequence alignment analyses, a potentially new immunomodulatory protein (GL18769) was expressed and shown to have high immunomodulatory activity. In this study, proteomic and biochemical analyses of G. lucidum were performed for the first time, revealing that proteins from this fungus can play significant bioactive roles and providing a new foundation for the further functional investigations that this fungus merits.
灵芝是一种担子菌纲白腐真菌，在世界范围内被用于药用。尽管最近已经报道了有关其基因组和转录组的信息，但在蛋白质组学水平上，关于灵芝的信息相对较少。在本研究中，制备了灵芝在三个发育阶段（16天的菌丝体以及60天和90天的子实体）的蛋白质组分，并进行了液相色谱-串联质谱（LC-MS/MS）分析。通过对灵芝基因组数据库进行检索，鉴定出803种蛋白质。在这些蛋白质中，检测到61种木质纤维素降解蛋白，其中大部分（49种蛋白）存在于90天的子实体中。还检测到14种与三羧酸循环相关的蛋白、17种肽酶、2种类AGO蛋白和2种免疫调节蛋白。803种蛋白质中的大多数（470种）具有基因本体（GO）注释，并被分类为36个GO术语，其中“结合”、“催化活性”和“水解酶活性”的百分比很高。此外，803种蛋白质中的357种被归入至少一个直系同源基因簇（COG）功能类别，并被分为22个COG分类。基于蛋白质组学和序列比对分析的结果，表达了一种潜在的新型免疫调节蛋白（GL18769），并显示其具有高免疫调节活性。在本研究中，首次对灵芝进行了蛋白质组学和生化分析，揭示了这种真菌的蛋白质可以发挥重要的生物活性作用，并为该真菌值得进一步进行的功能研究提供了新的基础。
