Kim Linda, Rao A Venket, Rao Leticia G
Department of Nutritional Sciences, Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada.
J Med Food. 2003 Summer;6(2):79-86. doi: 10.1089/109662003322233468.
We explored the possibility that lycopene, a carotenoid that is abundant in tomatoes, has effects on proliferation and differentiation of osteoblasts, the cells responsible for bone formation. Human osteoblast-like osteosarcoma SaOS-2 cells were cultured for 24 hours, after which varying doses of a water-dispersible microemulsion preparation of lycopene or vehicle of the same dilution were added. The cells were further cultured for 24 to 144 hours, and then the cell numbers were counted. Lycopene at 10(-6) and 10(-5) M had significant stimulatory effects on cell numbers, compared with the corresponding vehicle treatment, at all time points from 24 to 144 hours. The effects of lycopene on activity of the differentiation marker alkaline phosphatase activity in the absence or presence of dexamethasone were shown to be dependent on the stage of cell differentiation. This is the first report on the effects of lycopene on osteoblasts of human origin; the results may have important applications in the prevention of osteoporosis.
我们探讨了番茄红素（一种在番茄中大量存在的类胡萝卜素）对成骨细胞（负责骨形成的细胞）增殖和分化产生影响的可能性。将人成骨样骨肉瘤SaOS-2细胞培养24小时，之后添加不同剂量的番茄红素水分散性微乳制剂或相同稀释度的赋形剂。细胞进一步培养24至144小时，然后计数细胞数量。在24至144小时的所有时间点，与相应的赋形剂处理相比，10(-6)和10(-5) M的番茄红素对细胞数量具有显著的刺激作用。结果表明，在存在或不存在地塞米松的情况下，番茄红素对分化标志物碱性磷酸酶活性的影响取决于细胞分化阶段。这是关于番茄红素对人源成骨细胞影响的首次报道；这些结果可能在骨质疏松症的预防中具有重要应用。
