Ishii A, Kurihara R, Kojima T, Sano T, Mizuno Y, Yamakawa Y, Katsumata Y
Department of Legal Medicine and Bioethics, Nagoya University Graduate School of Medicine, Nagoya 464-8550, Japan.
Leg Med (Tokyo). 2000 Aug;2(2):115-8. doi: 10.1016/s1344-6223(00)80035-8.
Tetracyclic antidepressants, mianserin and setiptiline, in human body fluids have been found measurable with high sensitivity by gas chromatography (GC) with surface ionization detection (SID). The compounds in human whole blood or urine samples were extracted by solid-phase extraction using Bond Elut C(18) cartridges. The recoveries of both compounds from the body fluids were above 85%. For quantitation of mianserin, 25 ng of setiptiline was used as internal standard; and for quantitation of setiptiline, vice versa. The calibration curves for spiked whole blood or urine samples were linear in the range of 1.25-50 ng/ml. The detection limit of mianserin and setiptiline was about 1 ng/ml, which is comparable to those obtained by the previous GC-mass spectrometry methods. Our method seems very useful for determination of mianserin and setiptiline in forensic and clinical toxicology, because of high sensitivity, low background impurities and easy handling of the GC instrument.
已发现四环类抗抑郁药米安色林和塞替普汀在人体体液中可通过带表面电离检测（SID）的气相色谱法（GC）进行高灵敏度测定。人体全血或尿液样本中的化合物使用Bond Elut C(18)柱通过固相萃取进行提取。两种化合物从体液中的回收率均高于85%。对于米安色林的定量，使用25 ng塞替普汀作为内标；对于塞替普汀的定量，则反之。加标全血或尿液样本的校准曲线在1.25 - 50 ng/ml范围内呈线性。米安色林和塞替普汀的检测限约为1 ng/ml，这与先前的GC - 质谱法所获得的检测限相当。由于具有高灵敏度、低背景杂质以及气相色谱仪易于操作等特点，我们的方法对于法医和临床毒理学中米安色林和塞替普汀的测定似乎非常有用。
