Ishii A, Seno H, Suzuki O, Hattori H, Kumazawa T
Department of Legal Medicine, Hamamatsu University School of Medicine, Japan.
J Anal Toxicol. 1997 Jan-Feb;21(1):36-40. doi: 10.1093/jat/21.1.36.
A simple and sensitive method for determination of N,N-dimethyltryptamine (DMT) by gas chromatography (GC) with surface ionization detection (SID) is presented. Whole blood or urine, containing DMT and gramine (internal standard), was subjected to solid-phase extraction with a Sep-Pak C18 cartridge before analysis by GC-SID. The calibration curve was linear in the DMT range of 1.25-20 ng/mL blood or urine. The detection limit of DMT was about 0.5 ng/mL (10 pg on-column). The recovery of both DMT and gramine spiked in biological fluids was above 86%.
介绍了一种采用表面电离检测（SID）气相色谱法（GC）测定N，N - 二甲基色胺（DMT）的简单灵敏方法。含有DMT和禾本科碱（内标）的全血或尿液，在通过GC - SID分析之前，先用Sep - Pak C18柱进行固相萃取。校准曲线在DMT浓度为1.25 - 20 ng/mL血液或尿液范围内呈线性。DMT的检测限约为0.5 ng/mL（柱上10 pg）。生物流体中添加的DMT和禾本科碱的回收率均高于86%。
