Jin Zheng-Bin, Huang Xiu-Lin, Nakajima Yasuhiro, Yukawa Nobuhiro, Osawa Motoki, Takeichi Sanae
Department of Forensic Medicine, Tokai University School of Medicine, Kanagawa 259-1193, Isehara, Japan.
Leg Med (Tokyo). 2003 Jun;5(2):87-92. doi: 10.1016/s1344-6223(03)00042-7.
The present study analyses the human Y-chromosome minisatellite locus, MSY1 (DYF155S1), in 205 Japanese males of 191 pedigrees using the minisatellite variant repeat (MVR) mapping system. The internal haploid structures of the detected alleles considerably varied and consisted of three major repeat units: types 2, 3 and 4. A comparison of the haploid profiles of the MVR codes identified 185 distinct alleles, of which only five were shared. We did not detect a type 1 repeat unit, and variations were frequent at the 5' end of the minisatellite locus. Within an analysis of 24 paternally linked DNA samples donated by ten families, no mutational events were identified even over two generation gaps. Furthermore, we applied this mapping system to a paternity test in which the alleged father was missing.
本研究使用微卫星变异重复（MVR）图谱系统，对191个家系的205名日本男性的人类Y染色体微卫星位点MSY1（DYF155S1）进行了分析。检测到的等位基因的内部单倍体结构差异很大，由三个主要重复单元组成：2型、3型和4型。对MVR编码的单倍体图谱进行比较，鉴定出185个不同的等位基因，其中只有5个是共享的。我们未检测到1型重复单元，并且在微卫星位点的5'端变异频繁。在对十个家庭捐赠的24个父系连锁DNA样本的分析中，即使跨越两代间隔也未发现突变事件。此外，我们将此图谱系统应用于一名所谓父亲失踪的亲子鉴定。
