Jobling M A, Bouzekri N, Taylor P G
Department of Genetics, University of Leicester, Adrian Building, University Road, Leicester LE1 7RH, UK.
Hum Mol Genet. 1998 Apr;7(4):643-53. doi: 10.1093/hmg/7.4.643.
We describe the first haploid minisatellite, the human Y chromosome-specific locus, MSY1. It consists of an array of 48-114 AT-rich 25 bp repeats of at least five different variant types. A minisatellite variant repeat PCR (MVR-PCR) system gives Y-specific DNA codes, with a virtual heterozygosity of 99.9%, making MSY1 by far the single most variable locus on the Y. African populations contain the most diverged MSY1 structures. MSY1 is the only Y-chromosomal system where the characteristics of large numbers of mutations can be studied in detail: it provides a uniquely powerful tool both for the investigation of mutation in a haploid system, and for the dating of paternal lineages.
我们描述了首个单倍体微卫星——人类Y染色体特异性位点MSY1。它由48至114个富含AT的25 bp重复序列组成，至少有五种不同的变体类型。微卫星变体重复序列PCR（MVR-PCR）系统可产生Y特异性DNA编码，虚拟杂合度为99.9%，这使得MSY1成为Y染色体上迄今为止最具变异性的单一基因座。非洲人群中MSY1结构的差异最大。MSY1是唯一能详细研究大量突变特征的Y染色体系统：它为研究单倍体系统中的突变以及父系谱系的溯源提供了一个独特而强大的工具。
