采用全自动硅胶膜纯化技术从口腔拭子中提取基因组DNA用于法医分析。
Isolation of genomic DNA from buccal swabs for forensic analysis, using fully automated silica-membrane purification technology.
作者信息
Hanselle Thomas, Otte Michael, Schnibbe Thomas, Smythe Emma, Krieg-Schneider Frank
机构信息
QIAGEN GmbH, Max-Volmer Strasse 4, 40724 Hilden, Germany.
出版信息
Leg Med (Tokyo). 2003 Mar;5 Suppl 1:S145-9. doi: 10.1016/s1344-6223(02)00099-8.
Abstract
We developed a reliable and efficient, fully automated method for high-throughput isolation of genomic DNA from buccal swabs, based on silica-membrane technology. It was demonstrated that DNA purified using this method performed successfully in downstream applications relevant to forensic science and molecular diagnostics.
摘要
我们基于硅胶膜技术开发了一种可靠、高效的全自动方法，用于从口腔拭子中高通量分离基因组DNA。结果表明，使用该方法纯化的DNA在法医学和分子诊断相关的下游应用中表现良好。
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