Xiaohong Zhao, Xiaorui Chen, Jun Hu, Qisheng Qin
Department of Forensic Medicine, Tongji Medical College of Huazhong University of Science and Technology, Wuhan, People's Republic of China.
Leg Med (Tokyo). 2002 Mar;4(1):47-51. doi: 10.1016/s1344-6223(01)00054-2.
In this study, an animal model of early myocardial ischemia (EMI) was established by ligating the left anterior descending coronary artery of rats. The experimental animals were divided into five groups according to different intervals of MI (15, 30min, 1, 2, and 3h) and one control group. Tissues from the apex of the myocardium and the adjacent myocardium were taken for paraffin sections, followed by hematoxylin-eosin and streptavidin-biotin-peroxidase complex (SABC) staining. Results showed that the myoglobin (Mb) depletion and the fibrinogen (Fg) staining increase were detected in the 30min MI group. The wavy-like increasing extension of the size and the intensity of the Mb depletion and the Fg staining intensification from the subendocardial to the subepicardial cells were observed along with the prolongation of the ischemic period. Both changes had similar patterns and sensitivity, except Fg was less reliable than Mb as it is more easily contaminated by blood. After overcoming blood contamination, the SABC-Fg technique will provide a new method for the diagnosis of EMI.
在本研究中，通过结扎大鼠左冠状动脉前降支建立早期心肌缺血（EMI）动物模型。将实验动物根据心肌梗死不同时间间隔（15、30分钟、1、2和3小时）分为五组和一个对照组。取心肌心尖部及相邻心肌组织制作石蜡切片，随后进行苏木精-伊红染色和链霉亲和素-生物素-过氧化物酶复合物（SABC）染色。结果显示，在心肌梗死30分钟组检测到肌红蛋白（Mb）缺失和纤维蛋白原（Fg）染色增加。随着缺血时间延长，观察到Mb缺失的范围和强度呈波浪状增加扩展，以及Fg染色增强从心内膜下细胞延伸至心外膜下细胞。两种变化具有相似模式和敏感性，只是Fg比Mb可靠性稍差，因为它更容易被血液污染。克服血液污染后，SABC-Fg技术将为早期心肌缺血的诊断提供一种新方法。
