Václavek P, Koudela B, Modrý D, Sedlák K
Department of Parasitology, University of Veterinary and Pharmaceutical Sciences, Palackého 1-3, 612 42 Brno, Czech Republic.
Vet Parasitol. 2003 Jul 29;115(3):239-45. doi: 10.1016/s0304-4017(03)00215-2.
A serological survey for antibodies against Neospora caninum in aborting cattle was carried out in the Czech Republic. Serum samples from 463 aborting dairy cows originated from 137 farms from different parts of the Czech Republic were tested for presence of N. caninum antibodies by use of an enzyme-linked immunosorbent assay (ELISA) and an indirect fluorescent antibody test (IFAT). Antibodies (> or = 1:640) to N. caninum were found in 18 (3.9%) of 463 aborting cows. Farm prevalence in aborting cows was 12.4% (17/137). The antibody titres of cows were 1:200 (9 cows), 1:640 (7 cows), 1:1280 (3 cows), 1:2560 (3 cows), 1:5120 (3 cows), 1:10,240 (2 cows) and 1:20,480 (0 cow). A case-control study was conducted to estimate the association of N. caninum infection and abortion. For this 407 serum samples were collected from cows on five dairy farms with repeated occurrence of endemic and sporadic abortion of unidentified etiology. These samples were obtained from aborting cattle (n=44) and normally calving cattle (control group; n=363) and tested for N. caninum antibodies by an immunofluorescent antibody test (IFAT). Overall, 3.19% (13/407) of cows sampled had positive N. caninum fluorescence with a cut-off titre of 1:200. The prevalence of N. caninum was significantly higher (P<0.05) in the aborting group (13.64%; 95% confidence interval (CI): 5.2, 27.4) than in the control group (1.93%; 95% CI: 0.8, 3.9). A strong association between seropositivity and abortion was found, with seropositive cows being eight times more likely to abort than seronegative cows (odds ratio=8; 95% CI: 2.6, 25.1). This first report on the serological prevalence of N. caninum in cows in the Czech Republic verified a strong association between N. caninum infection and abortions in five dairy farms. Thus, the neosporosis should be considered in differential diagnosis of bovine abortion.
在捷克共和国对流产母牛进行了一项针对犬新孢子虫抗体的血清学调查。从捷克共和国不同地区137个农场的463头流产奶牛采集血清样本，采用酶联免疫吸附测定（ELISA）和间接荧光抗体试验（IFAT）检测犬新孢子虫抗体的存在情况。在463头流产母牛中，有18头（3.9%）检测到犬新孢子虫抗体（≥1:640）。流产母牛的农场患病率为12.4%（17/137）。母牛的抗体滴度分别为1:200（9头）、1:640（7头）、1:1280（3头）、1:2560（3头）、1:5120（3头）、1:10240（2头）和1:20480（0头）。开展了一项病例对照研究以评估犬新孢子虫感染与流产之间的关联。为此，从5个奶牛场反复发生病因不明的地方性和散发性流产的母牛中采集了407份血清样本。这些样本取自流产母牛（n = 44）和正常产犊母牛（对照组；n = 363），并通过免疫荧光抗体试验（IFAT）检测犬新孢子虫抗体。总体而言，在采样的母牛中，3.19%（13/407）的犬新孢子虫荧光检测呈阳性，截断滴度为1:200。流产组中犬新孢子虫的患病率（13.64%；95%置信区间（CI）：5.2，27.4）显著高于对照组（1.93%；95%CI：0.8，3.9）（P<0.05）。发现血清阳性与流产之间存在强关联，血清阳性的母牛流产的可能性是血清阴性母牛的8倍（优势比 = 8；95%CI：2.6，25.1）。这份关于捷克共和国母牛中犬新孢子虫血清学患病率的首份报告证实了5个奶牛场中犬新孢子虫感染与流产之间存在强关联。因此，在牛流产的鉴别诊断中应考虑新孢子虫病。
