Reddacliff L A, Vadali A, Whittington R J
Elizabeth Macarthur Agricultural Institute, New South Wales Agriculture, PMB 8, Camden, NSW, Australia.
Vet Microbiol. 2003 Sep 24;95(4):271-82. doi: 10.1016/s0378-1135(03)00181-0.
The effect of decontamination protocols on the numbers of sheep strain Mycobacterium avium subsp. paratuberculosis isolated in BACTEC cultures from clinical samples was assessed by spiking tissues and faeces at various points during the decontamination procedure. Routine protocols in the laboratory were shown to decrease the number of organisms isolated per sample by about 2.7 log(10) and 3.1 log(10) for faeces and tissues, respectively. These findings are important for the interpretation of negative culture results and may be useful in epidemiological studies. Addition of a centrifugation step to the tissue protocol increased the recovery by about 1 log, but resulted in increased contamination of BACTEC cultures. These studies may also facilitate future improvements to decontamination procedures.
通过在净化程序的不同阶段向组织和粪便中加入特定物质，评估了净化方案对从临床样本的BACTEC培养物中分离出的绵羊型副结核分枝杆菌数量的影响。结果表明，实验室的常规方案可使每份粪便样本和组织样本中分离出的微生物数量分别减少约2.7个对数（10）和3.1个对数（10）。这些发现对于解释培养阴性结果很重要，并且可能在流行病学研究中有用。在组织净化方案中增加离心步骤可使回收率提高约1个对数，但会导致BACTEC培养物污染增加。这些研究也可能有助于未来改进净化程序。
