Vilanova Lourdes T, Rauch M Cecilia, Mansilla Alejandra, Zambrano Angara, Brito Mónica, Werner Enrique, Alfaro Víctor, Cox José F, Concha Ilona I
Instituto de Bioquímica, Facultad de Ciencias, Universidad Austral de Chile, Campus Isla Teja, Valdivia, Chile.
Theriogenology. 2003 Oct 1;60(6):1083-95. doi: 10.1016/s0093-691x(03)00106-7.
The granulocyte-macrophage colony stimulating factor (GM-CSF) is a pleiotropic cytokine capable of stimulating proliferation, maturation and function of hematopoietic cells. Receptors for this cytokine are composed of two subunits, alpha and beta, and are expressed on myeloid progenitors and mature mononuclear phagocytes, monocytes, eosinophils and neutrophils, as well as in other nonhematopietic cells. We have recently demonstrated that bull spermatozoa express functional GM-CSF receptors that signal for increased glucose and Vitamin C uptake. In this study, we analyzed the expression of GM-CSF in bovine and human germ cells and its influence in bovine sperm motility. Reverse transcription-polymerase chain reaction (RT-PCR), in situ hybridization and immunoblotting analysis demonstrated that adult bovine and human testes expressed GM-CSF. In addition, immunolocalization studies confirmed the presence of GM-CSF in the germ cell line in bovine and human testes. Computer-assisted evaluation of patterns of sperm motility demonstrated that the addition of GM-CSF enhances several parameters of sperm motility in the presence of glucose or fructose substrates.
粒细胞-巨噬细胞集落刺激因子（GM-CSF）是一种多效性细胞因子，能够刺激造血细胞的增殖、成熟和功能。该细胞因子的受体由α和β两个亚基组成，在髓系祖细胞、成熟单核吞噬细胞、单核细胞、嗜酸性粒细胞和中性粒细胞以及其他非造血细胞上表达。我们最近证明，牛精子表达功能性GM-CSF受体，该受体可发出信号增加葡萄糖和维生素C的摄取。在本研究中，我们分析了GM-CSF在牛和人类生殖细胞中的表达及其对牛精子活力的影响。逆转录-聚合酶链反应（RT-PCR）、原位杂交和免疫印迹分析表明，成年牛和人类睾丸表达GM-CSF。此外，免疫定位研究证实了GM-CSF在牛和人类睾丸生殖细胞系中的存在。计算机辅助评估精子活力模式表明，在存在葡萄糖或果糖底物的情况下，添加GM-CSF可提高精子活力的几个参数。
