Restrepo M I, Velez J A, McElmeel M L, Whitney C G, Jorgensen J H
Department of Medicine, University of Texas Health Science Center, San Antonio, Texas 78229, USA.
Antimicrob Agents Chemother. 2003 Sep;47(9):2974-7. doi: 10.1128/AAC.47.9.2974-2977.2003.
Daptomycin MICs at which 50% of isolates were inhibited (MIC(50)s) and MIC(90)s determined by the NCCLS broth microdilution method were both 0.25 microg/ml (range, 0.06 to 2 microg/ml) for 350 pneumococcal isolates. MICs determined by E test strips on commercially prepared Mueller-Hinton sheep blood agars with different calcium contents were 2 to 3 dilutions higher than those determined by strips that contained daptomycin plus calcium. Daptomycin zone diameters varied little on the same media.
采用美国国家临床实验室标准化委员会(NCCLS)肉汤微量稀释法测定的350株肺炎球菌分离株的50%被抑制时的达托霉素最低抑菌浓度(MIC50)和MIC90均为0.25μg/ml(范围为0.06至2μg/ml)。在不同钙含量的商业制备的穆勒-欣顿绵羊血琼脂上,用E试验条测定的MIC比含有达托霉素加钙的试验条测定的结果高2至3个稀释度。在相同培养基上,达托霉素抑菌圈直径变化不大。