Fertilase激光系统与传统机械方法在卵胞浆内单精子注射中固定精子的应用。一项随机对照试验。

Application of the Fertilase-laser system versus the conventional mechanical method to immobilize spermatozoa for intracytoplasmic sperm injection. A randomized controlled trial.

作者信息

Debrock Sophie, Spiessens Carl, Afschrift Hilde, Van der Auwera Ingrid, D'Hooghe Thomas M

机构信息

Leuven University Fertility Center, UZ Gasthuisberg, Leuven, Belgium.

出版信息

Gynecol Obstet Invest. 2003;56(2):102-5. doi: 10.1159/000072995. Epub 2003 Aug 25.

DOI:10.1159/000072995

PMID:12937343

Abstract

A randomized controlled trial was set up to test the hypothesis that the fertilization rate of oocytes after intracytoplasmic sperm injection (ICSI) is higher after immobilization of the spermatozoa with the Fertilase-laser system technology than after immobilization of the spermatozoa with the conventional mechanical method. Metaphase II oocytes were injected with spermatozoa that were immobilized with the conventional mechanical method (group A, n=177) or with spermatozoa that were immobilized with the Fertilase-laser system technology (group B, n=179). The fertilization rate per successfully injected oocyte was comparable in group A (62.6%; 92/147) and in group B (56.3%; 89/158)(p=0.3). No difference could be observed in fertilization rates of oocytes injected with spermatozoa that were immobilized with the Fertilase-laser system technology compared to spermatozoa immobilized with the conventional mechanical method.

摘要

开展了一项随机对照试验，以检验以下假设：采用Fertilase激光系统技术使精子制动后进行卵胞浆内单精子注射（ICSI），其卵母细胞受精率高于采用传统机械方法使精子制动后的受精率。将处于中期II的卵母细胞分别与采用传统机械方法制动的精子（A组，n = 177）或采用Fertilase激光系统技术制动的精子（B组，n = 179）进行注射。成功注射的卵母细胞的受精率在A组（62.6%；92/147）和B组（56.3%；89/158）中相当（p = 0.3）。与采用传统机械方法制动的精子相比，采用Fertilase激光系统技术制动的精子注射的卵母细胞受精率未观察到差异。