Carvalho Ana Fontenele Urano, Melo Vânia Maria Maciel, Craveiro Afrânio Aragão, Machado Maria Iracema Lacerda, Bantim Márcia Braga, Rabelo Emanuelle Fontenele
Departamento de Biologia, Universidade Federal do Ceará, Campus do Pici 60455-900 Fortaleza, DE, Brasil.
Mem Inst Oswaldo Cruz. 2003 Jun;98(4):569-71. doi: 10.1590/s0074-02762003000400027. Epub 2003 Aug 18.
The aim of this work was to study the larvicidal activity of Lippia sidoides essential oil against Aedes aegypti larvae. The essential oil and its hydrolate (saturated solution of essential oil in water) were obtained by vapor extraction and their chemical composition determined by GL-chromatography coupled to mass spectroscopy. Bioassays were run with the essential oil, pure and diluted hydrolate and with their main constituents thymol and carvacrol. The results obtained showed that L. sidoides essential oil and its hydrolate have larvicidal action against the mosquito A. aegypti, causing an almost instantaneous mortality. Thymol, an alkylated phenol derivative and one of the major components of L. sidoides essential oil, was identified as the active principle responsible for the larvicidal action, causing 100% larval mortality at the lowest tested concentration of 0.017% (w/v). These results suggest that the essential oil of L. sidoides is promising as larvicide against A. aegypti and could be useful in the search of newer, more selective, and biodegradable larvicidal natural compounds to be used in official combat programs and at home.
本研究旨在探究南美牛至精油对埃及伊蚊幼虫的杀幼虫活性。通过水蒸气蒸馏法获得精油及其水解液（精油在水中的饱和溶液），并采用气相色谱-质谱联用技术测定其化学成分。使用精油、纯净及稀释后的水解液及其主要成分百里香酚和香芹酚进行生物测定。所得结果表明，南美牛至精油及其水解液对埃及伊蚊幼虫具有杀幼虫作用，可导致几乎瞬间死亡。百里香酚，一种烷基化酚类衍生物，也是南美牛至精油的主要成分之一，被确定为杀幼虫作用的活性成分，在最低测试浓度0.017%（w/v）时可导致100%的幼虫死亡。这些结果表明，南美牛至精油有望成为针对埃及伊蚊的杀幼虫剂，可用于寻找新型、更具选择性且可生物降解的杀幼虫天然化合物，以用于官方防治计划和家庭防治。
