Buckett William M
University of Liverpool, Liverpool Women's Hospital, Liverpool, UK.
Asian J Androl. 2003 Sep;5(3):209-12.
To determine the predictive value of the hypo-osmotic swelling (HOS) test to identify viable, non-motile sperm.
Semen samples from 20 men with severe asthenozoospermia underwent traditional seminal analysis, eosin-nigrosin (EN) staining and the HOS test. A further EN stain was then performed on a HOS pre-treated aliquot and a total of 2000 further sperm examined.
The median sperm density was 5.1 million/mL (IQR 4.3 approximately 13.1) and the median motility was 3.0% (IQR 0 approximately 7). Seven samples showed complete asthenozoospermia. Initial EN staining showed 59% viability (range 48 approximately 69) despite the poor standard parameters and 47% (range 33 approximately 61) in the complete asthenozoospermia subgroup. The HOS test showed 49.9% reacted overall (range 40 approximately 59) and 41.7% (range 22 approximately 61) in the complete asthenozoospermia subgroup. The combined HOS/EN stain showed the positive predictive value of the HOS test to identify viable sperm was 84.2 % overall and 79.7% in the complete asthenozoospermia subgroup.
The HOS test can effectively predict sperm viability in patients with severe and complete asthenozoospermia.
确定低渗肿胀（HOS）试验对识别有活力、无运动能力精子的预测价值。
对20例严重弱精子症男性的精液样本进行传统精液分析、伊红 - 黑色素（EN）染色和HOS试验。然后对经HOS预处理的一份样本进行进一步的EN染色，并对另外2000个精子进行检查。
精子密度中位数为510万/mL（四分位间距430万至1310万），运动能力中位数为3.0%（四分位间距0至7）。7份样本显示完全弱精子症。尽管标准参数不佳，但初始EN染色显示存活率为59%（范围48至69），在完全弱精子症亚组中为47%（范围33至61）。HOS试验显示总体反应率为49.9%（范围40至59），在完全弱精子症亚组中为41.7%（范围22至61）。联合HOS/EN染色显示HOS试验识别有活力精子的阳性预测值总体为84.2%，在完全弱精子症亚组中为79.7%。
HOS试验可有效预测严重和完全弱精子症患者的精子活力。
