Aribarg A, Sukcharoen N, Chanprasit Y, Ngeamvijawat J
Department of Obstetrics and Gynaecology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
J Med Assoc Thai. 1995 Sep;78(9):474-80.
To determine the relationships between pre-freeze semen variables and cryosurvival rate and post-thaw motility and examine whether they have any predictive value for the cryosurvival rate and post-thaw sperm motility, conventional semen analysis, supravital staining for sperm viability and hypo-osmotic swelling test were performed on 50 semen samples before cryopreservation. Thawed semen samples were examined for post-thaw sperm motility and cryosurvival rate. Significant correlations were observed between post-thaw sperm motility and several pre-freeze semen variables, such as, hypo-osmotic swelling test, pre-freeze sperm motility and sperm viability. In a stepwise regression analysis, an accurate prediction of post-thaw sperm motility (R = 0.826) was obtained using a multiple regression equation incorporating 3 variables including hypo-osmotic swelling test, pre-freeze sperm motility and sperm concentration. In conclusion, a set of criteria have been identified that accurately predicts post-thaw sperm motility and which place particular emphasis on hypo-osmotic swelling test. Conventional semen analysis and hypo-osmotic swelling test are simple and effective assays for the prediction of post-thaw motility.
为了确定冻前精液变量与冷冻存活率及解冻后活力之间的关系，并检验它们对冷冻存活率和解冻后精子活力是否具有预测价值，在50份精液样本冷冻保存前进行了常规精液分析、精子活力的体外活体染色及低渗肿胀试验。对解冻后的精液样本进行解冻后精子活力和冷冻存活率检测。观察到解冻后精子活力与几个冻前精液变量之间存在显著相关性，如低渗肿胀试验、冻前精子活力和精子存活率。在逐步回归分析中，使用包含低渗肿胀试验、冻前精子活力和精子浓度这3个变量的多元回归方程，获得了对解冻后精子活力的准确预测（R = 0.826）。总之，已确定了一组标准，可准确预测解冻后精子活力，且特别强调低渗肿胀试验。常规精液分析和低渗肿胀试验是预测解冻后活力的简单有效的检测方法。
