Lin Zhen, Chen Shiping, Ye Chuanzhong, Zhu Shaoxing
Department of Urology, Union Hospital Affiliated to Fujian Medical University, Fuzhou 350001, People's Republic of China.
Urol Res. 2003 Aug;31(4):232-5. doi: 10.1007/s00240-003-0302-9. Epub 2003 Feb 27.
Nitric oxide (NO) is synthesized by the enzyme family of nitric oxide synthases (NOS) and plays an important role in tumor growth and angiogenesis. The expression of two of the NOS isoforms, the endothelial and inducible isoforms (eNOS and iNOS, respectively), were evaluated in bladder tissue from patients with transitional cell carcinoma (TCC). The specimens were procured from 58 patients with TCC and 14 cases of normal bladder mucosa were used as a control group. NOS immunohistochemistry was performed and microvessal density (MVD) was determined. iNOS specific proteins were found in 47 of 58 bladder cancer specimens but not in control bladder tissue. The endothelial cells in both normal urothelium and tumor tissue showed a highly positive eNOS immunostaining. The MVD was 39.3+/-19.5 and 29.3+/-10.5 in TCC positive and negative for iNOS, respectively ( P<0.01). A correlation between iNOS immunoreactivity and tumor grade in bladder carcinoma could not be verified. These results indicate that NO generation from iNOS in the malignant epithelium and from eNOS in tumor stroma play a important role in tumor angiogenesis.
一氧化氮（NO）由一氧化氮合酶（NOS）家族的酶合成，在肿瘤生长和血管生成中起重要作用。评估了两种NOS亚型，即内皮型和诱导型亚型（分别为eNOS和iNOS）在移行细胞癌（TCC）患者膀胱组织中的表达。标本取自58例TCC患者，并以14例正常膀胱黏膜作为对照组。进行了NOS免疫组织化学检测并测定了微血管密度（MVD）。在58例膀胱癌标本中的47例中发现了iNOS特异性蛋白，但在对照膀胱组织中未发现。正常尿路上皮和肿瘤组织中的内皮细胞均显示eNOS免疫染色呈高度阳性。iNOS阳性和阴性的TCC中MVD分别为39.3±19.5和29.3±10.5（P<0.01）。无法证实膀胱癌中iNOS免疫反应性与肿瘤分级之间的相关性。这些结果表明，恶性上皮中的iNOS和肿瘤基质中的eNOS产生的NO在肿瘤血管生成中起重要作用。
