Tu Y, Liu L
Department of Chemistry and Chemical Engineering, Suzhou University, 215006 Suzhou.
Guang Pu Xue Yu Guang Pu Fen Xi. 2000 Dec;20(6):880-2.
This paper reports the assay of ofloxacin by fluorescence spectrophotometry. The fluorescence strength of ofloxacin is proportional to its concentration within the range from 0.500-25.0 ppm. The linear regression coefficient equals to 0.9999, and the recovery ratio is 99.0%. The results of determination for the samples of ofloxacin capsule from China and Japan are very close to that determined by the ultraviolet spectrophotometry, there isn't any obviously difference which was examined by Student's test. This method is both simplicity and accuracy, also it needs only one kind of reagent. It's believable that this method would be suitable for the use in clinical medicine and pharmaceutical industry.
本文报道了用荧光分光光度法测定氧氟沙星的方法。在0.500 - 25.0 ppm范围内，氧氟沙星的荧光强度与其浓度成正比。线性回归系数为0.9999，回收率为99.0%。中国和日本氧氟沙星胶囊样品的测定结果与紫外分光光度法测定的结果非常接近，经t检验无明显差异。该方法简便、准确，仅需一种试剂。相信该方法适用于临床医学和制药工业。
