Application of derivative UV spectrophotometry for the determination of ciprofloxacin, norfloxacin and ofloxacin in tablets.

The first-, second-, third- and fourth-order derivative spectrophotometric methods, by using the "peak-zero" (P-0) and "peak-peak" (P-P) techniques of measurement have been developed for the determination of ciprofloxacin hydrochloride, norfloxacin and ofloxacin in tablets. The calibration curves are linear within the concentration range of 2.0-12.0 micrograms ml-1 for ciprofloxacin hydrochloride, 1.0-10.0 micrograms ml-1 for norfloxacin and 2.5-15.0 micrograms ml-1 for ofloxacin. The procedure is simple, rapid and the results are reliable.