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通过流式细胞术检测白细胞介素-2对骨髓的激活作用。

Measurement of the activation of IL-2 on bone marrow by flow cytometry.

作者信息

Liu Z, You Y, Chen Z, Zou P

机构信息

Institute of Hematology, Xiehe Hospital, Tongji Medical University, Wuhan 430022.

出版信息

J Tongji Med Univ. 1999;19(4):264-6. doi: 10.1007/BF02886958.

Abstract

The changes of cell surface markers before and after activation by IL-2 were detected by flow cytometry (FCM) to establish a more convenient and precise criterion for the judgment of the activation of bone marrow. By using the measurement of the release of lactate dehydrogenase (LDH) the cytotoxicity of mononuclear cells (MNCs) from bone marrow, activated or inactivated, on tumor cell line K562 was evaluated, and at the same time the changes of surface markers on MNCs before and after activation were examined by using FCM. The results showed that the cytotoxicity of MNCs from bone marrow activated by IL-2 on tumor cell line K562 was increased obviously and the number of CD25+ and CD70+ positive cells in bone marrow MNCs was higher than before activation. The enhanced cytotoxicity of MNCs on tumor cell line K562 was synchronous with the increase of the number of CD25+ and CD70+ positive cells in 48 to 72 h. It is more direct, simple and precise to demonstrate the activation of IL-2 on bone marrow by detecting the changes of the amount of the CD25+ and CD70+ positive cells in bone marrow by FCM.

摘要

采用流式细胞术(FCM)检测IL-2激活前后细胞表面标志物的变化,以建立更便捷、精确的骨髓激活判断标准。通过检测乳酸脱氢酶(LDH)释放量,评估活化或未活化的骨髓单个核细胞(MNCs)对肿瘤细胞系K562的细胞毒性,同时利用FCM检测MNCs激活前后表面标志物的变化。结果显示,IL-2激活的骨髓MNCs对肿瘤细胞系K562的细胞毒性明显增强,骨髓MNCs中CD25+和CD70+阳性细胞数量高于激活前。在48至72小时内,MNCs对肿瘤细胞系K562细胞毒性的增强与CD25+和CD70+阳性细胞数量的增加同步。通过FCM检测骨髓中CD25+和CD70+阳性细胞数量的变化来证明IL-2对骨髓的激活作用更直接、简单且精确。

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