Yang F C, Agematsu K, Nakazawa T, Mori T, Ito S, Kobata T, Morimoto C, Komiyama A
Department of Pediatrics, Shinshu University School of Medicine, Matsumoto, Japan.
Immunology. 1996 Jun;88(2):289-93. doi: 10.1111/j.1365-2567.1996.tb00017.x.
The CD27 molecule is expressed on a portion of natural killer (NK) cells as well as T and B cells. To provide the functional capacity of CD27 molecule on NK cells, we here highly purified CD3- CD56+ NK cells by flow cytometry (purity > 98%), and investigated their NK cell activity via CD27/CD70 interaction using a CD70-transfectant by a 4 h 51Cr-release assay. The enhancement of NK activity by purified NK cells in the presence of interleukin-2 (IL-2) or interleukin-12 (IL-12) against CD70/Nalm-6 was not recognized as compared to against mock/Nalm-6. However, after a coculture with the fixed CD70/300-19, the purified NK cells increased the NK cell activity against K562, the value being 10 to 20% higher than coculture with the mock/300-19 in the presence of IL-2 or IL-12. The enhancement of NK activity was blocked by the addition of anti-CD70 monoclonal antibody (mAb). In addition, conjugation of NK cells to the target was increased by coculture with the CD70/300-19 without increased expression of adhesion molecules. In the parallel experiment, there was no increase in the killing capacity of NK cells. These results strongly show that CD27/CD70 interaction directly enhances NK activity in the presence of IL-2 or IL-12 by increasing the effector and target conjugate formation, indicating that CD27/CD70 interaction plays an important role in the cytotoxic function of NK cells.
CD27分子在一部分自然杀伤（NK）细胞以及T细胞和B细胞上表达。为了探究CD27分子在NK细胞上的功能，我们通过流式细胞术高度纯化了CD3-CD56+NK细胞（纯度>98%），并使用CD70转染细胞，通过4小时51Cr释放试验，研究了它们通过CD27/CD70相互作用产生的NK细胞活性。与mock/Nalm-6相比，在白细胞介素-2（IL-2）或白细胞介素-12（IL-12）存在的情况下，纯化的NK细胞对CD70/Nalm-6的NK活性增强未被识别。然而，与固定的CD70/300-19共培养后，纯化的NK细胞对K562的NK细胞活性增加，在IL-2或IL-12存在的情况下，该值比与mock/300-19共培养时高10%至20%。添加抗CD70单克隆抗体（mAb）可阻断NK活性的增强。此外，与CD70/300-19共培养可增加NK细胞与靶标的结合，而粘附分子的表达未增加。在平行实验中，NK细胞的杀伤能力没有增加。这些结果有力地表明，在IL-2或IL-12存在的情况下，CD27/CD70相互作用通过增加效应细胞与靶标的结合直接增强NK活性，表明CD27/CD70相互作用在NK细胞的细胞毒性功能中起重要作用。
