Avramescu Maria-Elena, Borneman Zandrie, Wessling Matthias
Membrane Technology Group, Faculty of Chemical Technology, University of Twente, P.O. Box 217, NL 7500 AE Enschede, The Netherlands.
J Chromatogr A. 2003 Jul 18;1006(1-2):171-83. doi: 10.1016/s0021-9673(03)00562-4.
The separation of two similarly sized proteins, bovine serum albumin (BSA) and bovine hemoglobin (Hb) was carried out using a new type of ion-exchange mixed-matrix adsorber membranes. The adsorber membranes were prepared by incorporation of various types of Lewatit ion-exchange resins into an ethylene-vinyl alcohol copolymer porous structure. The obtained heterogeneous matrices, composed of solid particles surrounded by the polymeric film, display high static and dynamic protein adsorption capacities. The effect of operational parameters such as filtration flow-rate, pH, and ionic strength on the protein separation performances was investigated for cation- as well as anion-exchange adsorber membranes. An average separation factor was calculated by numerical integration of the protein concentration in the permeate curve during the filtration run. High average separation factor values were obtained for BSA-Hb separation at physiological ionic strength with a filtration flow-rate up to 20 1/h per m2, until the protein breakthrough point at 10% of the feed concentration.
使用新型离子交换混合基质吸附膜对两种大小相似的蛋白质——牛血清白蛋白（BSA）和牛血红蛋白（Hb）进行了分离。通过将各种类型的Lewatit离子交换树脂掺入乙烯-乙烯醇共聚物多孔结构中来制备吸附膜。所获得的由被聚合物膜包围的固体颗粒组成的非均相基质表现出高的静态和动态蛋白质吸附容量。研究了诸如过滤流速、pH值和离子强度等操作参数对阳离子和阴离子交换吸附膜蛋白质分离性能的影响。通过在过滤过程中对渗透曲线中蛋白质浓度进行数值积分来计算平均分离因子。在生理离子强度下，以高达每平方米20升/小时的过滤流速进行BSA-Hb分离，直至达到进料浓度的10%的蛋白质突破点，获得了较高的平均分离因子值。
