Li Y, Spencer H G
Department of Chemistry, Clemson University, South Carolina 29634-1905.
J Biotechnol. 1992 Nov;26(2-3):203-11. doi: 10.1016/0168-1656(92)90007-v.
Anion exchange membranes prepared by adsorption of polymers on Formed-In-Place microfiltration substrates were formed and ion-exchange separations of solutions containing two proteins were determined by ion exchange membrane sequential separation procedures, similar to affinity membrane separation procedures. Representative ion exchange separation processes utilizing adsorbed poly(ethylene imine) (PEI) as the ion exchange membrane for the separation of the components of solutions containing two proteins, bovine serum albumin (BSA) and lysozyme and ovalbumin and lysozyme, are described. The stability of the PEI adsorbed layer, binding characteristics of the BSA to the membrane and purification properties of the procedure were determined.
通过在原位成型微滤基材上吸附聚合物制备的阴离子交换膜被制成，并且通过离子交换膜顺序分离程序测定含有两种蛋白质的溶液的离子交换分离，该程序类似于亲和膜分离程序。描述了利用吸附的聚（乙烯亚胺）（PEI）作为离子交换膜来分离含有两种蛋白质（牛血清白蛋白（BSA）和溶菌酶以及卵清蛋白和溶菌酶）的溶液组分的代表性离子交换分离过程。测定了PEI吸附层的稳定性、BSA与膜的结合特性以及该程序的纯化性能。
