Roussot Odile, Feve Katia, Plisson-Petit Florence, Pitel Frédérique, Faure Jean-Michel, Beaumont Catherine, Vignal Alain
Laboratoire de génétique cellulaire, Institut national de la recherche agronomique, 31326 Castanet-Tolosan, France.
Genet Sel Evol. 2003 Sep-Oct;35(5):559-72. doi: 10.1186/1297-9686-35-6-559.
The quail is a valuable farm and laboratory animal. Yet molecular information about this species remains scarce. We present here the first genetic linkage map of the Japanese quail. This comprehensive map is based solely on amplified fragment length polymorphism (AFLP) markers. These markers were developed and genotyped in an F2 progeny from a cross between two lines of quail differing in stress reactivity. A total of 432 polymorphic AFLP markers were detected with 24 TaqI/EcoRI primer combinations. On average, 18 markers were produced per primer combination. Two hundred and fifty eight of the polymorphic markers were assigned to 39 autosomal linkage groups plus the ZW sex chromosome linkage groups. The linkage groups range from 2 to 28 markers and from 0.0 to 195.5 cM. The AFLP map covers a total length of 1516 cM, with an average genetic distance between two consecutive markers of 7.6 cM. This AFLP map can be enriched with other marker types, especially mapped chicken genes that will enable to link the maps of both species and make use of the powerful comparative mapping approach. This AFLP map of the Japanese quail already provides an efficient tool for quantitative trait loci (QTL) mapping.
鹌鹑是一种有价值的农场和实验动物。然而，关于这个物种的分子信息仍然很少。我们在此展示日本鹌鹑的首张遗传连锁图谱。这张综合图谱仅基于扩增片段长度多态性（AFLP）标记构建。这些标记是在来自两个应激反应不同品系鹌鹑杂交产生的F2后代中开发并进行基因分型的。用24对TaqI/EcoRI引物组合共检测到432个多态性AFLP标记。平均每个引物组合产生18个标记。其中258个多态性标记被分配到39个常染色体连锁群以及ZW性染色体连锁群。连锁群包含2至28个标记，长度从0.0到195.5厘摩。AFLP图谱全长1516厘摩，相邻两个标记之间的平均遗传距离为7.6厘摩。这张AFLP图谱可以用其他标记类型丰富，特别是已定位的鸡基因，这将能够把两个物种的图谱联系起来，并利用强大的比较作图方法。这张日本鹌鹑的AFLP图谱已经为数量性状位点（QTL）作图提供了一个有效的工具。
