Huang Chang-Wen, Cheng Yu-Shin, Rouvier Roger, Yang Kuo-Tai, Wu Chean-Ping, Huang Hsiu-Lin, Huang Mu-Chiou
Department of Animal Science, National Chung Hsing University, 250 Kuo-Kung Road, Taichung, Taiwan.
Genet Sel Evol. 2009 Mar 17;41(1):28. doi: 10.1186/1297-9686-41-28.
Amplified fragment length polymorphism (AFLP) with multicolored fluorescent molecular markers was used to analyze duck (Anas platyrhynchos) genomic DNA and to construct the first AFLP genetic linkage map. These markers were developed and genotyped in 766 F2 individuals from six families from a cross between two different selected duck lines, brown Tsaiya and Pekin. Two hundred and ninety-six polymorphic bands (64% of all bands) were detected using 18 pairs of fluorescent TaqI/EcoRI primer combinations. Each primer set produced a range of 7 to 29 fragments in the reactions, and generated on average 16.4 polymorphic bands. The AFLP linkage map included 260 co-dominant markers distributed in 32 linkage groups. Twenty-one co-dominant markers were not linked with any other marker. Each linkage group contained three to 63 molecular markers and their size ranged between 19.0 cM and 171.9 cM. This AFLP linkage map provides important information for establishing a duck chromosome map, for mapping quantitative trait loci (QTL mapping) and for breeding applications.
采用带有多色荧光分子标记的扩增片段长度多态性技术（AFLP）分析鸭（绿头鸭）基因组DNA，并构建首张AFLP遗传连锁图谱。这些标记在来自两个不同选育鸭系（褐色菜鸭和北京鸭）杂交产生的6个家系的766个F2个体中进行开发和基因分型。使用18对荧光TaqI/EcoRI引物组合检测到296条多态性条带（占所有条带的64%）。每个引物组在反应中产生7至29条片段，平均产生16.4条多态性条带。AFLP连锁图谱包含260个共显性标记，分布在32个连锁群中。21个共显性标记未与任何其他标记连锁。每个连锁群包含3至63个分子标记，其大小在19.0厘摩至171.9厘摩之间。该AFLP连锁图谱为建立鸭染色体图谱、定位数量性状位点（QTL定位）和育种应用提供了重要信息。
