Ma P, Wu C Y
School of Pharmacy, West China University of Medical Sciences, Chengdu.
Yao Xue Xue Bao. 1992;27(10):763-7.
Anisodamine is a tropane alkaloid isolated from the plant Anisodus tanguticus (Maxim) Pasch. It is an anticholine drug widely used in clinics. Micellar liquid chromatography is a new type of HPLC developed in the 1980's. Direct plasma injection technique is the application of micellar HPLC in bioanalyses. In this paper, a micellar HPLC method, which employs n-propanol as modifier, SDS as surfactant, atropine sulphate as internal standard, has been developed. By direct injection, this method was successfully applied to the measurement of plasma level of anisodamine. Application of this method to the study of anisodamine pharmacokinetics was investigated in human volunteers following a single intramuscular injection. The separation was performed in a Shim-pack CLC-CN column (150 mm x 6 mm ID, 5 microns) with a mobile phase of n-propanol-water (15:85) with 45 mmol/L SDS and total ion strength 70 mmol/L by adding phosphate, and detected at 205 nm. The standard curve was linear over the concentration range of 15-750 ng plasma level (r = 0.9972). The measurable lowest concentration was 10 ng/ml plasma (S/N = 3:1). The study of anisodamine pharmacokinetic in man was also described.
山莨菪碱是从唐古特山莨菪（Maxim）Pasch植物中分离得到的一种托烷生物碱。它是一种在临床上广泛使用的抗胆碱能药物。胶束液相色谱法是20世纪80年代发展起来的一种新型高效液相色谱法。直接血浆进样技术是胶束高效液相色谱法在生物分析中的应用。本文建立了一种胶束高效液相色谱法，该方法以正丙醇为改性剂，十二烷基硫酸钠为表面活性剂，硫酸阿托品为内标。通过直接进样，该方法成功应用于山莨菪碱血浆浓度的测定。在人体志愿者单次肌肉注射后，研究了该方法在山莨菪碱药代动力学研究中的应用。分离在Shim-pack CLC-CN柱（150 mm×6 mm内径，5微米）上进行，流动相为正丙醇-水（15:85），含45 mmol/L十二烷基硫酸钠，通过添加磷酸盐使总离子强度为70 mmol/L，检测波长为205 nm。标准曲线在血浆浓度15 - 750 ng范围内呈线性（r = 0.9972）。可测最低浓度为血浆10 ng/ml（信噪比= 3:1）。还描述了山莨菪碱在人体药代动力学的研究。
