Holliday L S, Vakani A, Archer L, Dolce C
Department of Orthodontics, College of Dentistry, University of Florida, Box 100444, JHMHC, Gainesville, FL 32610-0444, USA.
J Dent Res. 2003 Sep;82(9):687-91. doi: 10.1177/154405910308200906.
Matrix metalloproteinases are involved in the regulation of bone remodeling. The hypothesis that matrix metalloproteinase inhibitors may be useful for experimentally limiting orthodontic tooth movement, a process involving perturbations of normal bone remodeling, was tested. General matrix metalloproteinase inhibitors limited the resorption of bone slices by mouse marrow cultures stimulated by calcitriol, parathyroid hormone, and basic-fibroblast growth factor. Pre-coating dentin slices with short arginine-glycine aspartic acid (RGD) peptides, but not arginine-glycine-glutamic acid (RGE) controls, restored bone resorption in the presence of matrix metalloproteinase inhibitors. Orthodontic tooth movement was inhibited by local delivery of Ilomastat, a general matrix metalloproteinase inhibitor, with the use of ethylene-vinyl-acetate (ELVAX) 40, a non-biodegradable, non-inflammatory sustained-release polymer. This study shows that orthodontic tooth movement can be inhibited with the use of matrix metalloproteinase inhibitors, and suggests a mechanistic link between matrix metalloproteinase activity and the production of RGD peptides.
基质金属蛋白酶参与骨重塑的调节。本研究对基质金属蛋白酶抑制剂可能有助于通过实验限制正畸牙齿移动这一假说进行了验证，正畸牙齿移动是一个涉及正常骨重塑受到干扰的过程。一般的基质金属蛋白酶抑制剂可限制由骨化三醇、甲状旁腺激素和碱性成纤维细胞生长因子刺激的小鼠骨髓培养物对骨切片的吸收。用短的精氨酸 - 甘氨酸 - 天冬氨酸（RGD）肽预包被牙本质切片，而非精氨酸 - 甘氨酸 - 谷氨酸（RGE）对照物，可在存在基质金属蛋白酶抑制剂的情况下恢复骨吸收。使用乙烯 - 醋酸乙烯酯（ELVAX）40（一种不可生物降解、无炎症的缓释聚合物）局部递送一般的基质金属蛋白酶抑制剂伊洛马司他，可抑制正畸牙齿移动。本研究表明，使用基质金属蛋白酶抑制剂可抑制正畸牙齿移动，并提示基质金属蛋白酶活性与RGD肽产生之间存在机制联系。
