人牙龈中多种树突状细胞（DC）亚群以及成熟DC与原位CD4 + T细胞的关联。

Multiple dendritic cell (DC) subpopulations in human gingiva and association of mature DCs with CD4+ T-cells in situ.

作者信息

Jotwani R, Cutler C W

机构信息

Department of Periodontics, 110 Rockland Hall, School of Dental Medicine, State University of New York at Stony Brook, Stony Brook, NY 11794-8703, USA.

出版信息

J Dent Res. 2003 Sep;82(9):736-41. doi: 10.1177/154405910308200915.

DOI:10.1177/154405910308200915

PMID:12939360

Abstract

Gingival epithelium is a site of active trafficking of Langerhans cells (LCs), while the lamina propria in chronic periodontitis (CP) contains CD83+ mature dendritic cells (mDCs) and CD4+ T-cells. The immune cells that contribute to the mDCs, and whether mDCs engage with T-cells in situ, are unclear. Using several immunohistochemical approaches, combined with fluorescence-, light-, and scanning laser confocal-microscopy, we show that, in addition to LCs, the gingiva contains dermal DCs (DDCs) in the lamina propria; moreover, DDCs increase in number during CP. Furthermore, DDCs, LCs, and B-cells co-express CD83 in CP and contribute to the mDC pool. Double-staining for CD83 and CD4 revealed that mDCs associate with clusters of CD4+ T-cells in the lamina propria. Analysis of these data suggests that multiple DC subsets mature in the gingiva and that mature DCs engage in antigen presentation with T-cells in chronic periodontitis.

摘要

牙龈上皮是朗格汉斯细胞（LCs）活跃迁移的部位，而慢性牙周炎（CP）中的固有层含有CD83 +成熟树突状细胞（mDCs）和CD4 + T细胞。促成mDCs的免疫细胞以及mDCs是否在原位与T细胞相互作用尚不清楚。使用几种免疫组织化学方法，结合荧光显微镜、光学显微镜和扫描激光共聚焦显微镜，我们发现，除了LCs外，牙龈固有层中还含有真皮树突状细胞（DDCs）；此外，在CP期间DDCs的数量增加。此外，在CP中，DDCs、LCs和B细胞共表达CD83，并促成mDC池。CD83和CD4的双重染色显示，mDCs与固有层中的CD4 + T细胞簇相关联。对这些数据的分析表明，多个DC亚群在牙龈中成熟，并且成熟的DCs在慢性牙周炎中与T细胞进行抗原呈递。