Semerikov V, Lagercrantz U, Tsarouhas V, Rönnberg-Wästljung A, Alström-Rapaport C, Lascoux M
Department of Conservation Biology and Genetics, Evolutionary Biology Centre, Uppsala University, Norbyvägen 18 D, 75236 Uppsala, Sweden.
Heredity (Edinb). 2003 Sep;91(3):293-9. doi: 10.1038/sj.hdy.6800327.
A total of 88 selective primer combinations were screened using bulked males and females sampled from four families of Salix viminalis. A total of more than 1000 polymorphic fragments was obtained, of which only four cosegregated with sex. These four sex-linked markers were subsequently scored in individuals that were used for bulked sample preparation in additional individuals of the same families, and in individuals in other families. A pair of primers that amplified the sex-linked fragments was constructed from one of the sex-linked amplified fragment length polymorphism (AFLP) fragments. In hybridization of Southern blot filters with the sex-linked DNA fragments, the band was present in females and absent in males, but the opposite pattern of band segregation (a band found in males and no band in females) was never observed in either the AFLP or RFLP experiments. Two of the sex-linked markers were placed on a linkage map. They both map at the same location in a linkage group comprising other markers not segregating with sex. Our data suggest that a single locus governs the sex determination and that nonrecombining sex chromosomes are absent in S. viminalis. A close association was found between skewed sex ratio and segregation distortion at this locus.
使用从4个垂柳（Salix viminalis）家系中采集的雄性和雌性混合样本，共筛选了88种选择性引物组合。共获得了1000多个多态性片段，其中只有4个与性别共分离。随后，在用于制备同一家庭其他个体混合样本的个体以及其他家庭的个体中，对这4个性别连锁标记进行了评分。从其中一个性别连锁扩增片段长度多态性（AFLP）片段构建了一对扩增性别连锁片段的引物。在用性别连锁DNA片段杂交Southern杂交膜时，该条带在雌性中存在而在雄性中不存在，但在AFLP或RFLP实验中从未观察到相反的条带分离模式（条带在雄性中存在而在雌性中不存在）。其中两个性别连锁标记被定位到一个连锁图谱上。它们都定位在一个连锁群中的同一位置，该连锁群包含其他不与性别分离的标记。我们的数据表明，单个基因座控制性别决定，并且垂柳中不存在重组性染色体。在这个基因座上，发现偏态性别比与分离畸变之间存在密切关联。
