沙棘（沙棘属）雄性和雌性花芽中性别偏向的时间基因表达
Sex-Biased Temporal Gene Expression in Male and Female Floral Buds of Seabuckthorn (Hippophae rhamnoides).
作者信息
Chawla Aseem, Stobdan Tsering, Srivastava Ravi B, Jaiswal Varun, Chauhan Rajinder S, Kant Anil
机构信息
Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, Solan, India.
Defence Institute of High Altitude Research, Defence R & D Organisation, Leh, Jammu, and Kashmir, India.
出版信息
PLoS One. 2015 Apr 27;10(4):e0124890. doi: 10.1371/journal.pone.0124890. eCollection 2015.
Seabuckthorn is an economically important dioecious plant in which mechanism of sex determination is unknown. The study was conducted to identify seabuckthorn homologous genes involved in floral development which may have role in sex determination. Forty four putative Genes involved in sex determination (GISD) reported in model plants were shortlisted from literature survey, and twenty nine seabuckthorn homologous sequences were identified from available seabuckthorn genomic resources. Of these, 21 genes were found to differentially express in either male or female flower bud stages. HrCRY2 was significantly expressed in female flower buds only while HrCO had significant expression in male flowers only. Among the three male and female floral development stages (FDS), male stage II had significant expression of most of the GISD. Information on these sex-specific expressed genes will help in elucidating sex determination mechanism in seabuckthorn.
沙棘是一种具有重要经济价值的雌雄异株植物，其性别决定机制尚不清楚。本研究旨在鉴定参与花发育的沙棘同源基因，这些基因可能在性别决定中发挥作用。通过文献调研，从模式植物中报道的44个推测的性别决定基因（GISD）中筛选出候选基因，并从现有的沙棘基因组资源中鉴定出29个沙棘同源序列。其中，21个基因在雄花芽或雌花芽阶段差异表达。HrCRY2仅在雌花芽中显著表达，而HrCO仅在雄花中显著表达。在三个雌雄花发育阶段（FDS）中，雄性II期大多数GISD有显著表达。这些性别特异性表达基因的信息将有助于阐明沙棘的性别决定机制。
Zotero 插件