Taylor Jennifer L, Wu Chin-Lee, Cory David, Gonzalez R Gilberto, Bielecki Anthony, Cheng Leo L
Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Magn Reson Med. 2003 Sep;50(3):627-32. doi: 10.1002/mrm.10562.
The development of high-resolution magic angle spinning (HR-MAS) NMR spectroscopy for intact tissue analysis and the correlations between the measured tissue metabolites and disease pathologies have inspired investigations of slow-spinning methodologies to maximize the protection of tissue pathology structures from HR-MAS centrifuging damage. Spinning sidebands produced by slow-rate spinning must be suppressed to prevent their complicating the spectral region of metabolites. Twenty-two human prostatectomy samples were analyzed on a 14.1T spectrometer, with HR-MAS spinning rates of 600 Hz, 700 Hz, and 3.0 kHz, a repetition time of 5 sec, and employing various rotor-synchronized suppression methods, including DANTE, WATERGATE, TOSS, and PASS pulse sequences. Among them, DANTE, as the simplest scheme, has shown the most potential in suppression of tissue water signals and spinning sidebands, as well as in quantifying metabolic concentrations.
用于完整组织分析的高分辨率魔角旋转（HR-MAS）核磁共振波谱技术的发展，以及所测组织代谢物与疾病病理学之间的相关性，激发了人们对慢速旋转方法的研究，以最大程度地保护组织病理结构免受HR-MAS离心损伤。慢速旋转产生的旋转边带必须被抑制，以防止它们使代谢物的光谱区域复杂化。在一台14.1T的光谱仪上对22个前列腺切除的人体样本进行了分析，HR-MAS的旋转速率为600Hz、700Hz和3.0kHz，重复时间为5秒，并采用了各种转子同步抑制方法，包括DANTE、WATERGATE、TOSS和PASS脉冲序列。其中，DANTE作为最简单的方案，在抑制组织水信号和旋转边带以及定量代谢物浓度方面显示出最大潜力。
