Zhou Miao-Ping, Ren Li-Juan, Zhang Xu, Scholten Olga E, Shen Xiao-Rong, Bai Gui-Hua, Ma Hong-Xiang, Lu Wei-Zhong
Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.
Yi Chuan Xue Bao. 2003 Jun;30(6):571-6.
Three recombinant inbred populations, Ning894037/Alondra, Wangshuibai/Alondra and Sumai3/Alondra, were analyzed for QTLs associated with Fusarium head blight resistance by interval mapping and composite interval mapping in this study. The result showed that the major QTLs were detected on the short arm of chromosome 3B of all three resistant parents using the data of FHB resistance evaluated in greenhouse or field. They were located in the interval of 5.0 cM between BARC133 and Xgwm493 in Ning894037, 11.5 cM between BARC147 and Xgwm493 in Wangshuibai, and 13.0 cM between Xgwm533a and Xgwm493 in Sumai3, explaining 42.8%, 15.1% and 10.6% of the phenotypic variance for Type II resistance (spread within the spike), respectively. Some of the SSR markers linking to the major QTLs tightly can be used directly in marker-assisted breeding to improve FHB resistance in wheat.
本研究通过区间作图和复合区间作图,对三个重组自交系群体Ning894037/Alondra、Wangshuibai/Alondra和Sumai3/Alondra进行了与赤霉病抗性相关的QTL分析。结果表明,利用温室或田间评估的赤霉病抗性数据,在所有三个抗性亲本的3B染色体短臂上检测到主要QTL。它们在Ning894037中位于BARC133和Xgwm493之间5.0 cM的区间内,在Wangshuibai中位于BARC147和Xgwm493之间11.5 cM的区间内,在Sumai3中位于Xgwm533a和Xgwm493之间13.0 cM的区间内,分别解释了II型抗性(穗内扩展)表型变异的42.8%、15.1%和10.6%。一些与主要QTL紧密连锁的SSR标记可直接用于标记辅助育种,以提高小麦的赤霉病抗性。