Ma Mei, Rao Kaifeng, Wang Zijian
State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, PR China.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2003 Sep;38(9):1781-92. doi: 10.1081/ese-120022878.
Potential mutagenicities of water and sediment in Guanting Reservoir/Yongdinghe River Watershed in Beijing, China were assessed using the Ame's tests for the organic pollutants. For different sampling sites, the results were indicative for the existence of mutagens. Furthermore, by using different types of Ames tests, different types of mutagens in water and in sediment were elucidated. The results on Ames tests for water concentrates and sediment extracts could be obviously differentiated. Mutagens in water were mainly direct-acting mutagens whereas in sediment they were mainly pro-mutagens, which exhibit mutagenic effects only after metabolic activation. In general, among all the six sampling sites, site-1, site-2 and site-4 were potentially at mutagenic risk. In a trial to find the causes of mutagenicities in the sediment samples, chemical analysis was performed for polychlorinated biphenyls (PCBs) and organochlorinated pesticides. However, the available data were not sufficient to explain the mutagenicity in the sediment. Integration of the results of Ames test and chemical analysis on overlying water and sediment samples in Guanting Reservoir and Yongdinghe River indicated that this catchment was moderately contaminated by PCBs and organochlorinated pesticides thereby causing a significant mutaginicity in water and sediment. Special attention should be given to the water quality in site-1 and sediment quality in site-4.
采用Ames试验对中国北京官厅水库/永定河流域水体及沉积物中的有机污染物潜在致突变性进行了评估。针对不同采样点，结果表明存在诱变剂。此外，通过使用不同类型的Ames试验，阐明了水体和沉积物中不同类型的诱变剂。水体浓缩物和沉积物提取物的Ames试验结果有明显差异。水中的诱变剂主要是直接作用诱变剂，而沉积物中的主要是前诱变剂，前诱变剂只有在代谢活化后才表现出诱变作用。总体而言，在所有六个采样点中，1号点、2号点和4号点存在潜在诱变风险。为找出沉积物样品中致突变性的原因，对多氯联苯（PCBs）和有机氯农药进行了化学分析。然而，现有数据不足以解释沉积物中的致突变性。对官厅水库和永定河上覆水体及沉积物样品的Ames试验结果与化学分析结果进行整合，表明该流域受到多氯联苯和有机氯农药的中度污染，从而导致水体和沉积物中出现显著的致突变性。应特别关注1号点的水质和4号点的沉积物质量。
