Salamunić Ilza, Juretić Dubravka, Ljutić Dragan
Department of Laboratory Diagnostics, Split Clinical Hospital, Split, Croatia.
Clin Chem Lab Med. 2003 Jul;41(7):904-7. doi: 10.1515/CCLM.2003.137.
The main objective of the present study was to analyze some aspects of the function and structure of erythrocytes with respect to hemodialysis membrane biocompatibility throughout the erythrocyte and serum antioxidant levels. The study included 36 hemodialysis patients (14 female and 22 male, age 22-79 years, median 55) treated at the Department of Nephrology and Dialysis, Split Clinical Hospital in Split, and 30 control subjects matched for age and sex. Hemodialysis was performed three times a week for 4 hours with cellulose diacetate (n = 17; 6 females and 11 males) or polysulfone (n = 19; 8 females and 11 males) membranes. The aim of the study was to assess the level of oxidative stress in these patients by measuring catalytic concentrations of superoxide dismutase and glutathione peroxidase in erythrocyte lysate and scavenger systems related to free hemoglobin in serum (haptoglobin, hemopexin and bilirubin). In comparison with control values, the mean catalytic concentrations of superoxide dismutase were increased and catalytic concentrations of glutathione peroxidase decreased in patients before hemodialysis irrespective of the membrane used. Immediately after hemodialysis with either membrane, the mean catalytic concentrations of superoxide dismutase returned to the control range, while those of glutathione peroxidase were still decreased compared to control values, without any significant difference between the cellulose diacetate and polysulfone membranes. The predialysis and postdialysis values of haptoglobin, hemopexin and bilirubin in patient sera were within the range of control values. Comparison of the cellulose diacetate and polysulfone membranes showed no significant differences in the erythrocyte content of antioxidant enzymes and the scavenger system related to free hemoglobin in serum before and after hemodialysis.
本研究的主要目的是通过红细胞和血清抗氧化剂水平，分析红细胞功能和结构与血液透析膜生物相容性相关的某些方面。该研究纳入了36例在斯普利特临床医院肾脏病与透析科接受治疗的血液透析患者（14例女性和22例男性，年龄22 - 79岁，中位数55岁），以及30例年龄和性别匹配的对照受试者。使用醋酸纤维素膜（n = 17；6例女性和11例男性）或聚砜膜（n = 19；8例女性和11例男性）每周进行3次、每次4小时的血液透析。本研究旨在通过测量红细胞裂解液中超氧化物歧化酶和谷胱甘肽过氧化物酶的催化浓度以及血清中与游离血红蛋白相关的清除系统（触珠蛋白、血红素结合蛋白和胆红素），评估这些患者的氧化应激水平。与对照值相比，无论使用何种膜，血液透析前患者中超氧化物歧化酶的平均催化浓度升高，而谷胱甘肽过氧化物酶的催化浓度降低。使用任一膜进行血液透析后，超氧化物歧化酶的平均催化浓度立即恢复到对照范围，而谷胱甘肽过氧化物酶的催化浓度与对照值相比仍降低，醋酸纤维素膜和聚砜膜之间无显著差异。患者血清中触珠蛋白、血红素结合蛋白和胆红素的透析前和透析后值均在对照值范围内。醋酸纤维素膜和聚砜膜的比较显示，血液透析前后红细胞抗氧化酶含量和血清中与游离血红蛋白相关的清除系统无显著差异。
