Trammell Brian C, Ma Lianjia, Luo Hao, Hillmyer Marc A, Carr Peter W
Department of Chemistry, University of Minnesota, 207 Pleasant Street SE, Minneapolis, MN 55455-0431, USA.
J Am Chem Soc. 2003 Sep 3;125(35):10504-5. doi: 10.1021/ja036339d.
We report a new reversed phase liquid chromatography (RPLC) phase with remarkable acid stability using dimethyl(ethylphenylchloromethyl)chlorosilane (1), oligomeric polystyrene (PS), and octylbenzene (C8). This phase Si-1-PS-C8 was prepared using silica modification processes and Friedel-Crafts alkylation chemistry. Under highly aggressive mobile phase conditions, Si-1-PS-C8 exhibited remarkable stability as evinced by only minimal reduction in retention factor (k') after 1400 column volumes at pH = 0.5 and 150 degrees C. The peak shapes for a variety of basic solutes were symmetric using Si-1-PS-C8. Evidence for a highly cross-linked coating of the silica particles was observed using scanning electron microscopy. The remarkable stability of this phase is unparalleled as compared to all other RPLC phases reported to date.
