利用微阵列鉴定特定病原体-free虾中白斑综合征病毒潜伏相关基因。 （注：这里“specific-pathogen-free”直译为“无特定病原体”，根据语境推测这里可能是想说“无特定病原体的虾”，所以译文补充了“虾”字使表达更完整）
Identification of white spot syndrome virus latency-related genes in specific-pathogen-free shrimps by use of a microarray.
作者信息
Khadijah Siti, Neo Soek Ying, Hossain M S, Miller Lance D, Mathavan S, Kwang Jimmy
机构信息
Animal Health Biotechnology, Temasek Life Sciences Laboratory, 1 Research Link, The National University of Singapore, Singapore 117604.
出版信息
J Virol. 2003 Sep;77(18):10162-7. doi: 10.1128/jvi.77.18.10162-10167.2003.
Abstract
To investigate whether specific-pathogen-free (SPF) shrimps are asymptomatic carriers of white spot syndrome virus (WSSV), we used a WSSV-specific DNA microarray to measure WSSV gene expression in SPF and WSSV-infected shrimps. Three WSSV genes were found to be relatively highly expressed in SPF shrimps. Reverse transcription-PCR using nested primers as well as real-time detection confirmed that these genes have no detectable counterparts in GenBank; structural analysis of the putative proteins revealed helix-loop-helix and leucine zipper motifs. Viral sequences could be PCR amplified from genomic DNA of SPF shrimp, further supporting the suggestion that these shrimps are asymptomatic carriers.
摘要
为了研究无特定病原体（SPF）虾是否为白斑综合征病毒（WSSV）的无症状携带者，我们使用了一种WSSV特异性DNA微阵列来测量SPF虾和感染WSSV的虾中WSSV基因的表达。我们发现有三个WSSV基因在SPF虾中相对高表达。使用巢式引物的逆转录PCR以及实时检测证实，这些基因在GenBank中没有可检测到的对应序列；对推定蛋白质的结构分析揭示了螺旋-环-螺旋和亮氨酸拉链基序。病毒序列可从SPF虾的基因组DNA中通过PCR扩增得到，这进一步支持了这些虾是无症状携带者的观点。
相似文献
1
Identification of white spot syndrome virus latency-related genes in specific-pathogen-free shrimps by use of a microarray.
J Virol. 2003 Sep;77(18):10162-7. doi: 10.1128/jvi.77.18.10162-10167.2003.
2
Identification of two major virion protein genes of white spot syndrome virus of shrimp.
Virology. 2000 Jan 20;266(2):227-36. doi: 10.1006/viro.1999.0088.
3
Characterization of an early gene (wsv477) from shrimp white spot syndrome virus (WSSV).
Virus Genes. 2007 Apr;34(2):193-8. doi: 10.1007/s11262-006-0053-0. Epub 2006 Dec 1.
4
Characterization of fossilized relatives of the White Spot Syndrome Virus in genomes of decapod crustaceans.
BMC Evol Biol. 2015 Jul 19;15:142. doi: 10.1186/s12862-015-0380-7.
5
Identification of structural proteins from shrimp white spot syndrome virus (WSSV) by 2DE-MS.
Proteins. 2004 May 1;55(2):229-35. doi: 10.1002/prot.10640.
6
Transcriptional profile of shrimp white spot syndrome virus (WSSV) genes with DNA microarray.
Arch Virol. 2006 Sep;151(9):1723-33. doi: 10.1007/s00705-006-0751-x. Epub 2006 Apr 3.
7
Identification and phylogeny of a non-specific endonuclease gene of white spot syndrome virus of shrimp.
Virus Genes. 2001 Dec;23(3):331-7. doi: 10.1023/a:1012529524663.
8
The white spot syndrome virus DNA genome sequence.
Virology. 2001 Jul 20;286(1):7-22. doi: 10.1006/viro.2001.1002.
9
Transcriptional analysis of the ribonucleotide reductase genes of shrimp white spot syndrome virus.
Virology. 2000 Nov 10;277(1):92-9. doi: 10.1006/viro.2000.0596.
10
Transcriptional analysis of the DNA polymerase gene of shrimp white spot syndrome virus.
Virology. 2002 Sep 15;301(1):136-47. doi: 10.1006/viro.2002.1536.
引用本文的文献
1
Viruses, cancer and non-self recognition.
Open Biol. 2021 Mar;11(3):200348. doi: 10.1098/rsob.200348. Epub 2021 Mar 31.
2
An Elegant Analysis of White Spot Syndrome Virus Using a Graphene Oxide/Methylene Blue based Electrochemical Immunosensor Platform.
Sci Rep. 2017 Apr 10;7:46169. doi: 10.1038/srep46169.
3
Role of Litopenaeus vannamei Yin Yang 1 in the Regulation of the White Spot Syndrome Virus Immediate Early Gene .
J Virol. 2017 Feb 28;91(6). doi: 10.1128/JVI.02314-16. Print 2017 Mar 15.
4
Gene expression profiling in gill tissues of White spot syndrome virus infected black tiger shrimp Penaeus monodon by DNA microarray.
Virusdisease. 2015 Jun;26(1-2):9-18. doi: 10.1007/s13337-014-0243-7. Epub 2015 Feb 10.
5
Characterization of fossilized relatives of the White Spot Syndrome Virus in genomes of decapod crustaceans.
BMC Evol Biol. 2015 Jul 19;15:142. doi: 10.1186/s12862-015-0380-7.
6
Transcript Analysis of White spot syndrome virus Latency and Phagocytosis Activating Protein Genes in Infected Shrimp (Penaeus monodon).
Indian J Virol. 2012 Dec;23(3):333-43. doi: 10.1007/s13337-012-0119-7. Epub 2012 Nov 27.
7
Biology, Host Range, Pathogenesis and Diagnosis of White spot syndrome virus.
Indian J Virol. 2012 Sep;23(2):161-74. doi: 10.1007/s13337-012-0079-y. Epub 2012 Aug 14.
8
Transcriptome analysis on Chinese shrimp Fenneropenaeus chinensis during WSSV acute infection.
PLoS One. 2013;8(3):e58627. doi: 10.1371/journal.pone.0058627. Epub 2013 Mar 19.
9
Shrimp molecular responses to viral pathogens.
Mar Biotechnol (NY). 2011 Aug;13(4):587-607. doi: 10.1007/s10126-010-9287-x.
10
Microarray analyses of shrimp immune responses.
Mar Biotechnol (NY). 2011 Aug;13(4):629-38. doi: 10.1007/s10126-010-9291-1.
本文引用的文献
1
Estimation of prevalence of white spot syndrome virus (WSSV) by polymerase chain reaction in Penaeus monodon postlarvae at time of stocking in shrimp farms of Karnataka, India: a population-based study.
Dis Aquat Organ. 2002 Jun 3;49(3):235-43. doi: 10.3354/dao049235.
2
Detection of white spot syndrome virus (WSSV) of shrimp by means of monoclonal antibodies (MAbs) specific to an envelope protein (28 kDa).
Dis Aquat Organ. 2002 Apr 24;49(1):11-8. doi: 10.3354/dao049011.
3
Complete genome sequence of the shrimp white spot bacilliform virus.
J Virol. 2001 Dec;75(23):11811-20. doi: 10.1128/JVI.75.23.11811-11820.2001.
4
The latency-associated nuclear antigen encoded by Kaposi's sarcoma-associated herpesvirus activates two major essential Epstein-Barr virus latent promoters.
J Virol. 2001 Oct;75(19):9446-57. doi: 10.1128/JVI.75.19.9446-9457.2001.
5
The white spot syndrome virus DNA genome sequence.
Virology. 2001 Jul 20;286(1):7-22. doi: 10.1006/viro.2001.1002.
6
White spot syndrome virus (WSSV) in cultured Penaeus monodon in the Philippines.
Dis Aquat Organ. 2000 Aug 10;42(1):77-82. doi: 10.3354/dao042077.
7
A new bacterial white spot syndrome (BWSS) in cultured tiger shrimp Penaeus monodon and its comparison with white spot syndrome (WSS) caused by virus.
Dis Aquat Organ. 2000 May 25;41(1):9-18. doi: 10.3354/dao041009.
8
Natural and experimental infection of white spot syndrome virus (WSSV) in benthic larvae of mud crab Scylla serrata.
Dis Aquat Organ. 2000 Mar 14;40(2):157-61. doi: 10.3354/dao040157.
9
Evidence for the presence of a low-level, persistent baculovirus infection of Mamestra brassicae insects.
J Gen Virol. 1997 Jul;78 ( Pt 7):1801-5. doi: 10.1099/0022-1317-78-7-1801.
10
Amplification of mRNA populations using aRNA generated from immobilized oligo(dT)-T7 primed cDNA.
Biotechniques. 1996 Apr;20(4):584-91. doi: 10.2144/19962004584.
Zotero 插件