Lan Y, Xu X, Yang F, Zhang X
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, SOA, Xiamen, P.R. China.
Arch Virol. 2006 Sep;151(9):1723-33. doi: 10.1007/s00705-006-0751-x. Epub 2006 Apr 3.
In an attempt to obtain the transcriptional profile of shrimp white spot syndrome virus (WSSV) genes, DNA microarray analysis was performed using amplified DNA fragments of the WSSV genome that covered most of the presumptive open reading frames (ORFs). Total RNAs were extracted from WSSV-infected crayfish (Cambarus clarkii) and reverse transcribed into cDNA and labeled with 32P-dATP. The DNA microarray detected 81.1% of the ORFs, and 47 of these were transcribed at 6 h post infection, suggesting they may be early genes. Some early genes were further confirmed by temporal analyses of gene transcription. Results in this study indicated the effectiveness of DNA microarray for discovering early viral genes.
为了获得虾白斑综合征病毒（WSSV）基因的转录谱，使用覆盖了大部分推定开放阅读框（ORF）的WSSV基因组扩增DNA片段进行了DNA微阵列分析。从感染WSSV的小龙虾（克氏原螯虾）中提取总RNA，反转录成cDNA并用32P-dATP进行标记。DNA微阵列检测到81.1%的ORF，其中47个在感染后6小时转录，表明它们可能是早期基因。通过基因转录的时间分析进一步证实了一些早期基因。本研究结果表明DNA微阵列在发现病毒早期基因方面的有效性。
