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印度凡纳滨对虾浓核症病毒(PmDNV)的全核酸序列。

Complete nucleic acid sequence of Penaeus monodon densovirus (PmDNV) from India.

机构信息

Department of Fishery Microbiology, Karnataka Veterinary, Animal and Fisheries Sciences, University, College of Fisheries, Mangalore-575 002, India.

出版信息

Virus Res. 2010 Jun;150(1-2):1-11. doi: 10.1016/j.virusres.2010.02.005. Epub 2010 Feb 13.

Abstract

The complete nucleic acid sequence of the Penaeus monodon densovirus (PmDNV) from India was characterized. Analysis of the whole genome, consisting of 6310 bp revealed the presence of three open reading frames (ORFs), comprising 1281 bp, 1734 bp and 2460 bp, respectively. The complete genome and amino acid sequences of three proteins viz., NS1, NS2 and VP were compared with PmDNV from Thailand, PmergDNV from Australia and other partial sequences in GenBank, respectively. Highest nucleotide similarity was observed with the Thai strain (88%), while 33, 32 and 91 amino acid substitutions were observed in the NS2, NS1 and VP, respectively. Phylogenetic analysis of shrimp, insect and vertebrate parvovirus sequences revealed that the Indian PmDNV is more closely related to Thai isolates than all other parvoviruses reported so far.

摘要

对印度凡纳滨对虾浓核病毒(PmDNV)的完整核酸序列进行了特征分析。对全长 6310bp 的基因组分析显示,存在三个开放阅读框(ORFs),分别由 1281bp、1734bp 和 2460bp 组成。对完整基因组和三种蛋白质(NS1、NS2 和 VP)的氨基酸序列分别与来自泰国的 PmDNV、来自澳大利亚的 PmergDNV 和 GenBank 中的其他部分序列进行了比较。与泰国株的核苷酸相似性最高(88%),而 NS2、NS1 和 VP 中分别观察到 33、32 和 91 个氨基酸取代。对虾、昆虫和脊椎动物细小病毒序列的系统进化分析表明,与迄今为止报道的所有其他细小病毒相比,印度 PmDNV 与泰国分离株的亲缘关系更为密切。

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